Soil Bacteria-Enriching Agents for Microbiome Dysbiosis

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Current agriculture practices lead to decreased diversity of soil microorganisms and dysbiosis of crop microbiomes, necessitating sustainable solutions to enhance soil fertility and reduce reliance on harmful agrochemicals.

Innovation Solution

The method involves selecting carbon substrates and chemoattractants that specifically enrich desired bacterial species in the soil microbiome, such as PGPR, by manipulating the chemical composition of root exudates and applying exogenous soil prebiotics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If large quantities of chemical fertilizers and pesticides are applied to stimulate crop productivity, then crop yields increase, but environmental degradation occurs and downstream ecological harm increases

Engineering Contradiction:
Improvecrop productivityVSAvoidenvironmental degradation
Core Design Contradiction:
ProductivityVSObject-affected harmful factors

Solution Approach 1:

The patent uses plant growth-promoting rhizobacteria (PGPR) as intermediary organisms that naturally enhance crop productivity through biological mechanisms such as nitrogen fixation, phosphate solubilization, and phytohormone production, replacing the need for chemical fertilizers and pesticides while avoiding environmental degradation

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent manipulates the chemical composition of root exudates by introducing specific carbon substrates and chemoattractants, changing the nutritional parameters of the rhizosphere to selectively enrich desired PGPR species that provide beneficial functions for plant growth

Inventive Principle:
Principle #35Parameter changes

2Productivity

If monoculture crop systems and tillage farming are used to increase agricultural efficiency, then crop production increases, but soil microorganism diversity decreases and microbiome dysbiosis occurs

Engineering Contradiction:
Improveagricultural efficiencyVSAvoidsoil microorganism diversity
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent applies selective carbon substrates and chemoattractants locally in the rhizosphere zone to create micro-niches that favor specific beneficial PGPR species, allowing diversity enhancement in the soil microbiome while maintaining agricultural productivity in the crop system

Inventive Principle:
Principle #3Local quality

Solution Approach 2:

The patent pre-manipulates the chemical composition of root exudates before PGPR inoculation by adding specific carbon sources and chemoattractants, creating a favorable environment in advance that ensures successful colonization and establishment of desired bacterial species

Inventive Principle:
Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach effectively enriches the concentration of desired bacteria in the soil microbiome, improving nutrient use efficiency, pathogen resistance, and plant growth promotion, thereby enhancing agricultural productivity sustainably.

Implementation Method 1

selecting one or more chemoattractants that are able to attract a desired bacterial species to the soil microbiome

Methodology Applied
Scientific EffectChemotaxis:

Implementation Method 2

selecting one or more carbon substrates that are able to enrich a desired bacterial species in a soil microbiome

Methodology Applied
Scientific EffectChemical enrichment:

Data Source

PatentUS20250187998A1Identification of soil bacteria-enriching agents and methods of use thereof
Publication Date: 2025.06.12 CALIFORNIA INST OF TECH
  • US20250187998A1 patent drawing
  • US20250187998A1 patent drawing
  • US20250187998A1 patent drawing

AI summary

Some aspects of the present disclosure relate to methods and devices for identifying soil bacteria-enriching agents that selectively enrich a desired species of bacteria in a soil microbiome. In some embodiments, the soil bacteria-enriching agent comprises a carbon substrate and/or a chemoeffector. Some aspects of the present disclosure relate to methods of enriching a desired species of bacteria in a soil microbiome using one or more soil bacteria-enriching agents.