Solid Bacterial Composition for Agricultural Use

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Solution Overview

Problem

Current biopesticide and biofertilizer formulations, particularly those involving strain C-924, face challenges such as low stability, wettability issues, and lump formation, which hinder their effective application in agricultural and veterinary contexts, leading to reduced efficacy against plant and animal pathogens.

Innovation Solution

A solid composition comprising up to 92.4% bacterial concentrate of strain C-924, a culture medium, 1.8% to 6.6% antifoam substance, and 0.8% to 3% sucrose, with less than 12% residual moisture, optimized for improved stability and wettability, facilitating easier application and enhanced biopesticidal and biofertilizing properties.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If a solid formulation is used to improve stability, then storage stability is improved, but wettability deteriorates causing lump formation

Engineering Contradiction:
Improvestorage stabilityVSAvoidwettability
Core Design Contradiction:
Stability of the object's compositionVSEase of operation

Solution Approach 1:

A surfactant is introduced as an intermediary substance to mediate between the hydrophobic powder particles and the aqueous application medium. The surfactant molecules adsorb at the particle surface, providing hydrophilic groups that facilitate wetting and dispersion, thereby resolving the contradiction between maintaining solid formulation stability and achieving good wettability for application

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The surface properties of the powder particles are modified by changing the chemical composition parameters - specifically by incorporating surfactants and adjusting the ratio of hydrophobic to hydrophilic components in the formulation. This parameter change enables the particles to maintain structural integrity while improving their interaction with water for spray application

Inventive Principle:
Principle #35Parameter changes

2Manufacturing precision

If high agitation is used to improve mixing, then homogeneity is improved, but air incorporation increases leading to poor wettability

Engineering Contradiction:
ImprovehomogeneityVSAvoidwettability
Core Design Contradiction:
Manufacturing precisionVSEase of operation

Solution Approach 1:

The surfactant acts as a mediator that reduces surface tension and facilitates the release of trapped air bubbles from the powder matrix. By lowering the interfacial tension between air, water, and solid particles, the surfactant enables easier air evacuation and improves subsequent wettability, resolving the contradiction between achieving homogeneous mixing and maintaining good wetting properties

Inventive Principle:
Principle #24Intermediary (Mediator)

3Stability of the object's composition

If high viscosity is used to improve product stability, then formulation stability is improved, but air release becomes difficult reducing wettability

Engineering Contradiction:
Improveformulation stabilityVSAvoidair release
Core Design Contradiction:
Stability of the object's compositionVSEase of operation

Solution Approach 1:

The surfactant serves as a lubricating intermediary that reduces the interfacial adhesion between air bubbles and the viscous formulation matrix. By adsorbing at the air-formulation interface, the surfactant lowers the energy required for air bubble detachment and rise, enabling effective air release even in high viscosity formulations while maintaining formulation stability

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The rheological parameters of the formulation are optimized by adjusting the surfactant concentration and selecting appropriate surfactant types that provide the right balance between viscosity and air release. This parameter optimization allows the formulation to maintain sufficient viscosity for stability while enabling adequate air bubble mobility and escape

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The optimized solid composition maintains stability, reduces wettability time, and enhances soil fertility, effectively controlling nematodes and phytopathogenic fungi, while promoting plant growth and protecting animals from gastrointestinal zoonematodes, with demonstrated efficacy in both laboratory and field conditions.

Implementation Method 1

from 1.8% to 6.6% of an antifoam substance

Methodology Applied
Scientific EffectAntifoam: Antifoam

Implementation Method 2

from 0.8% to 3% sucrose

Methodology Applied
Scientific EffectSolvation: Solvation

Implementation Method 3

with less than 12% residual moisture

Methodology Applied
Scientific EffectEvaporation: Evaporation

Data Source

PatentEP3858141A1Solid composition for agricultural and veterinary use
Publication Date: 2021.08.04 CENT DE ING GENETICA & BIOTECNOLOGIA
  • EP3858141A1 patent drawingFigure 1~2
  • EP3858141A1 patent drawingFigure 3~4
  • EP3858141A1 patent drawing

AI summary

Solid composition for agricultural or veterinary use comprising a mixture of bacterial concentrate of the strain C-924 and a commercially available culture medium or organic amendment, an antifoaming substance, sucrose, and having less than 12% residual moisture. The components of the formulation allow an adequate wettability of the solid final product, and its prolonged stability at storage temperature of 2 to 8 °C. The invention discloses the use of the solid composition in the control of pathogens of plants and animals, and in the stimulation of seed germination and plant growth.