Solid Carrier Spot Transfection for Multiplexed Protein Delivery
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Solution Overview
Problem
Current methods for transfecting proteins and nucleic acid molecules into cells are inefficient and lack scalability, particularly for simultaneous and multiplexed transfections, which are essential for advanced cell biological assays and high-throughput applications.
Innovation Solution
A method involving a solid carrier with spots containing a transfection reagent, antibodies or their fragments, and nucleic acid molecules, where the transfection mixture is dried or frozen for storage and application, allowing for efficient and simultaneous transfection of multiple proteins and nucleic acids into cells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional transfection methods (microinjection, electroporation) are used to deliver proteins and nucleic acids into cells, then transfection can be achieved, but the methods are either not scalable, require intensive training, or have low efficiency for simultaneous multiplexed transfections
Solution Approach 1:
The transfection mixture is segmented into discrete spots on a solid carrier substrate, with each spot containing specific combinations of transfection reagents, proteins, and nucleic acids. This segmentation enables simultaneous multiplexed transfections of different cell lines with different molecular payloads in a single experiment, dramatically improving productivity while maintaining procedural simplicity.
Solution Approach 2:
A solid carrier substrate serves as an intermediary between the transfection reagents/proteins/nucleic acids and the target cells. The substrate is coated with the transfection mixture, allowing controlled interaction with cells without requiring complex delivery devices or procedures. This intermediary approach simplifies the transfection process while enabling high-throughput applications.
2Adaptability or versatility
If multiple transfection reagents, proteins, and nucleic acids are mixed together in solution for simultaneous transfection, then multiplexing is possible, but the mixture becomes complex and difficult to manage and store
Solution Approach 1:
The complex transfection mixture is segmented into discrete spots on a solid carrier, with each spot containing specific combinations of reagents, proteins, and nucleic acids. This physical segmentation maintains the versatility of simultaneous multiplexed transfections while organizing the complex components into manageable, addressable units that are easier to prepare, store, and reproduce.
Solution Approach 2:
The transfection mixture is transitioned from a three-dimensional liquid solution to a two-dimensional solid surface array. This dimensional change allows multiple transfection formulations to coexist in a single plane without mutual interference, simplifying storage and handling while preserving the ability to perform multiplexed transfections by selecting specific spots.
Data Source
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AI summary
The present invention relates to a method for solid phase transfection of at least one protein and at least one nucleic acid molecule into at least one cell comprising the step of providing a solid carrier containing at least one spot comprising a transfection reagent, at least one protein and the at least one nucleic acid molecule as well as to a solid carrier containing at least one spot comprising a transfection reagent, at least one protein and the at least one nucleic acid molecule.