Solid Matrix Nucleic Acid Separation Using Low Solubility Removing Agent
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Solution Overview
Problem
Current methods for separating nucleic acids from mixtures with solid matrices face challenges in efficiency and effectiveness, particularly in minimizing nucleic acid loss and rehydration, especially when dealing with low concentrations and dilutions.
Innovation Solution
A method and system utilizing a target compound removing agent with specific solubility properties to selectively remove target compounds from a solid matrix while retaining nucleic acids, allowing for effective separation and purification of nucleic acids at low concentrations and dilutions, using a solid matrix configured to absorb nucleic acids and interact with the removing agent.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of substance
If conventional solid matrix separation methods are used to separate nucleic acids from mixtures, then separation is achieved, but nucleic acid loss and rehydration occur, especially problematic at low concentrations
Solution Approach 1:
The patent changes the chemical parameters of the removing agent by selecting compounds with specific water solubility characteristics (equal to or less than 0.05 g per 100 mL at 25°C). This parameter change allows the agent to effectively remove target compounds while minimizing nucleic acid loss and rehydration, particularly beneficial for low concentration samples
Solution Approach 2:
The patent introduces a target compound removing agent as an intermediary substance that mediates between the solid matrix and the target compounds. This intermediary selectively interacts with target compounds to facilitate their removal while leaving nucleic acids bound to the solid matrix, thereby improving separation efficiency without increasing nucleic acid loss
2Loss of substance
If conventional separation methods are used, then target compounds are removed, but nucleic acid rehydration occurs which reduces purification quality
Solution Approach 1:
The patent modifies the chemical parameters of the removing agent to have low water solubility (equal to or less than 0.05 g per 100 mL at 25°C). This parameter change prevents water from being introduced to the solid matrix during the removal process, thereby minimizing nucleic acid rehydration and maintaining high purification quality
3Reliability
If standard separation procedures are applied to low concentration nucleic acid samples, then separation is performed, but effectiveness decreases due to minimal nucleic acid presence
Solution Approach 1:
The patent changes the concentration threshold parameter for effective separation by using removing agents with water solubility equal to or less than 0.05 g per 100 mL. This parameter change enables effective separation even when nucleic acid concentration is at low levels (1 μM or less), as the low-water-solubility agent minimizes both nucleic acid loss and rehydration
Solution Approach 2:
The patent employs a target compound removing agent as an intermediary that selectively binds to and removes target compounds without interfering with low concentration nucleic acids. This intermediary approach enhances separation effectiveness by providing a selective mechanism that works efficiently even when nucleic acid quantities are minimal
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The approach minimizes nucleic acid loss and rehydration, achieving high retention rates of nucleic acids on the solid matrix while effectively removing target compounds, enabling efficient separation and purification even at low concentrations and dilutions, and is compatible with various biochemical reactions.
Implementation Method 1
a solid matrix configured to absorb a nucleic acid
Implementation Method 2
contacting the solid matrix with a target compound removing agent... to remove the target compound from the solid matrix
Data Source
AI summary
Methods and systems and related compositions for separating through a solid matrix a mixture comprising a nucleic acid in a concentration of 1 μM or less, together with a target compound having a water solubility equal to or greater than 0.001 g per 100 mL, which can be used for managing fluid flow, biochemical reactions and purification of the nucleic acid or other target analytes. The method comprises contacting the solid matrix with a target compound removing agent having a water solubility equal to or less than 0.05 g per 100 mL at 25° C. at 1 atm pressure with water having a solubility in the removing agent of less than 10 g per 100 mL at 25° C. at 1 atm pressure, the contacting performed to remove the target compound from the solid matrix.