Solid-Phase Carrier for Affinity Chromatography
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Solution Overview
Problem
Affinity chromatography faces challenges in achieving high dynamic binding capacity and minimizing non-specific adsorption of impurities, particularly host cell proteins, while maintaining excellent antifouling properties.
Innovation Solution
A solid-phase carrier with a cyclic ether group and specific functional groups such as sulfinyl, sulfide, or oxy groups is developed, which is produced by methods involving thioglycerol treatment and sulfoxidation, enhancing the carrier's ability to immobilize ligands with high capacity and reduced impurity adsorption.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional solid-phase carriers are used for affinity chromatography, then ligand immobilization can be achieved, but non-specific adsorption of impurities occurs and dynamic binding capacity is limited
Solution Approach 1:
The patent applies parameter changes by modifying the chemical composition of the solid-phase carrier through specific monomer ratios (cross-linkable vinyl monomer with hydroxyl group: 10-50 wt%, epoxy group-containing non-cross-linkable vinyl monomer: 30-70 wt%). This chemical parameter optimization resolves the contradiction by enhancing both dynamic binding capacity and antifouling properties simultaneously, eliminating non-specific adsorption while maintaining high ligand immobilization capacity.
Solution Approach 2:
The patent employs composite materials by creating a copolymer structure combining two specific vinyl monomers with complementary functions. The cross-linkable vinyl monomer provides structural integrity and ligand binding sites, while the epoxy group-containing monomer contributes to antifouling properties. This composite approach resolves the technical contradiction by integrating multiple functions into a single material system that achieves both high productivity and low harmful factors.
2Reliability
If agarose particles are used as solid-phase carrier, then natural affinity properties are obtained, but manufacturing complexity and consistency control become issues
Solution Approach 1:
The patent replaces the mechanical/biological complexity of natural agarose particles with a chemically defined synthetic polymer system. Instead of relying on natural agarose extraction and processing, the invention uses controlled polymerization of specific vinyl monomers to create carriers with consistent, reproducible affinity properties. This substitution resolves the contradiction by maintaining reliability through chemical precision while eliminating manufacturing complexity associated with natural product processing.
3Productivity
If porous particles are used to increase surface area, then dynamic binding capacity improves, but non-specific adsorption of impurities increases
Solution Approach 1:
The patent applies local quality by creating different functional zones within the porous particle structure. The polymer composition is designed so that the pore surfaces and internal structures exhibit specific chemical properties (hydrophilicity, charge distribution) that promote selective ligand-target interactions while repelling non-specific impurity adsorption. This local optimization of chemical properties within the porous structure resolves the contradiction by enabling high surface area utilization without proportionally increasing harmful non-specific adsorption.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The resulting solid-phase carrier exhibits high dynamic binding capacity and excellent antifouling properties, effectively reducing non-specific adsorption of impurities like host cell proteins, thereby improving the efficiency of affinity chromatography.
Implementation Method 1
reacting the functional group of the scaffold with a reagent comprising a reactive group
Implementation Method 2
a group having a sulfinyl group, a sulfide group, an oxy group, or an imino group
Data Source
Figure 1
Figure 2
AI summary
There are provided a solid-phase carrier and a carrier for affinity refining that exhibit high dynamic binding capacity when a ligand is immobilized, have excellent antifouling properties, and are unlikely to have non-specific adsorption of impurities. The solid-phase carrier has a functional group capable of fixing a ligand and is characterized by having a group having a sulfinyl group, a sulfide group, an oxy group or an imino group, and a hydroxyl group, a thiol group, an amino group, a carboxy group, a sulfate group, a phosphate group or an alkanoyl group.