Solid-Phase Cartridge for LC-GC Organic Analysis
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
The combination of liquid chromatography and gas chromatography for analyzing organic chemical substances is hindered by the presence of water in elutes from liquid chromatography, preventing direct injection into gas chromatography.
Innovation Solution
A method involving fractionation of organic chemical substances by liquid chromatography, followed by adsorption onto a solid-phase cartridge and direct elution into a gas chromatography storage chamber, with optional derivatization using a reagent, allows for precise analysis without water and polar solvents, utilizing a syringe needle for injection and a switching valve for efficient operation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If liquid chromatography is used to extract and fractionate organic chemical substances, then analysis precision is improved, but the elute contains water and polar solvents that prevent direct injection into gas chromatography
Solution Approach 1:
A solid-phase cartridge is introduced as an intermediary device between the liquid chromatography elute and the gas chromatography injection system. The cartridge adsorbs organic chemical substances from the aqueous elute, concentrating them in an organic solvent phase that is compatible with gas chromatography. This mediator removes water and polar solvents while preserving the analytes for subsequent GC analysis.
Solution Approach 2:
The organic chemical substances are extracted from the aqueous elute using the solid-phase cartridge, which selectively adsorbs the target compounds while allowing water and polar solvents to pass through or be removed. This extraction step separates the harmful water content from the analytes, enabling compatible injection into the gas chromatography system.
2Ease of operation
If a solid-phase cartridge is used to adsorb and concentrate analysis target substances, then injection compatibility with gas chromatography is improved, but device complexity increases
Solution Approach 1:
The solid-phase cartridge unit is merged with the liquid chromatography system, allowing the cartridge to be positioned inline in the elute flow path. This integration combines the LC fractionation capability with the SP cartridge concentration function in a single continuous flow path, reducing the need for separate manual transfer steps and minimizing overall system complexity.
Solution Approach 2:
The solid-phase cartridge performs preliminary concentration and purification of the analysis target substances before they reach the gas chromatography injection system. By pre-concentrating the analytes in an organic solvent phase, the system avoids the need for complex post-processing steps and ensures direct compatibility with GC injection requirements.
3Measurement precision
If derivatization reagent is added to the eluent, then analysis range and accuracy are improved, but operational complexity increases
Solution Approach 1:
The derivatization reagent is merged with the eluent stream, allowing automatic mixing as the elute passes through the solid-phase cartridge. This integration ensures that derivatization occurs in-line during the concentration process, eliminating the need for separate derivatization steps and reducing operational complexity while improving analysis accuracy.
Solution Approach 2:
The derivatization process continues simultaneously with the concentration and purification steps, as the derivatization reagent is added to the eluent that is already being processed through the solid-phase cartridge. This continuous action ensures that all analytes are derivatized and concentrated in a single uninterrupted flow, simplifying operations and improving accuracy.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables high-precision, quick analysis of organic chemical substances, particularly for 1 to 20 sorts, by eliminating the need for water and polar solvents, simplifying the injection process, and reducing operational complexity, while enhancing the range and accuracy of analysis.
Implementation Method 1
continuously adsorbing said analysis target substance onto a solid-phase cartridge from the liquid chromatograph
Implementation Method 2
subjecting a sample for analysis prepared beforehand by extracting organic chemical substances from a target sample to fractionation by a liquid chromatograph
Data Source
AI summary
A combination of a liquid chromatograph and a gas chromatograph was a possible measure for improving precision in analyzing organic chemical substances. However, because elutes from liquid chromatoghaphies contain water and a highly polar solvent, injection thereof into a gas chromatograph has been impossible. Consequently, to develop an analytical method which realizes that combination and an apparatus thereof has been a subject. The method of the quantitative analysis comprises subjecting a sample for analysis prepared beforehand by extracting organic chemical substances from an assay sample to fractionation by a liquid chromatograph, continuously adsorbing a fractionated elute containing a substance to be determined onto a solid-phase cartridge while conducting the fractionation, eluting this substance, which has been adsorbed on the solid-phase cartridge, with an eluent, and transferring the elute to a storage chamber of a gas chromatograph.