Solid Phase Mixture for Selective Vitamin D3 Extraction
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Solution Overview
Problem
Current methods for detecting and quantifying vitamin D metabolites, particularly 1α,25-dihydroxyvitamin D3, are limited by slow processing times, high costs, and complexity, making further speedup, simplification, and cost reduction difficult.
Innovation Solution
A solid phase mixture containing an oxidizing agent, such as periodic acid, and solid phase particles like diatomaceous earth or silica gel, which selectively binds and oxidizes 1,2-diol compounds, allowing for the extraction of vitamin D metabolites, including 1α,25-dihydroxyvitamin D3, by distinguishing between 1,2-diol and 1,3-diol structures.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional immunoextraction or periodic acid treatment methods are used, then vitamin D metabolite detection can be performed, but processing time is excessive and costs are high
Solution Approach 1:
The invention divides the extraction process into two distinct functional components: a solid phase material for selective binding of 1α,25-dihydroxyvitamin D3 and its metabolites, and periodic acid for selective oxidation of interfering 1,2-diol compounds. This segmentation allows simultaneous achievement of rapid extraction and selective cleanup without requiring sequential complex procedures.
Solution Approach 2:
The invention creates a composite extraction system combining organic polymer particles (polystyrene, polymethacrylate, or polyvinyl alcohol) with periodic acid. This composite material integrates the adsorption capability of the polymer with the oxidative cleanup function of periodic acid, enabling both target analyte retention and interference removal in a single integrated medium that reduces processing time.
2Ease of manufacture
If conventional methods are used, then vitamin D metabolite extraction is achieved, but the procedure is complex and costly
Solution Approach 1:
The invention merges the target analyte binding function and the interference removal function into a single integrated extraction step using the composite material of polymer particles with periodic acid. This eliminates the need for separate binding and cleanup steps required in conventional immunoextraction methods, significantly simplifying the overall procedure.
Solution Approach 2:
The invention employs disposable polymer-based solid phase materials that can be used in single-use extraction cartridges or columns. These inexpensive, readily available polymer particles with periodic acid provide a cost-effective alternative to expensive antibody-based immunoextraction systems, reducing both material costs and procedural complexity.
3Measurement precision
If selective oxidation of 1,2-diol compounds is performed, then contaminants like 4β,25-dihydroxyvitamin D3 are removed, but the oxidizing agent must be carefully controlled
Solution Approach 1:
The invention applies local quality by incorporating periodic acid specifically within or on the surface of the polymer particle matrix at controlled concentrations (0.1-10% w/w). This localized placement ensures that oxidation occurs only where needed - at the solid-liquid interface where interfering 1,2-diol compounds contact the material - while protecting the target 1α,25-dihydroxyvitamin D3 from unwanted oxidation.
Solution Approach 2:
The invention optimizes the concentration parameter of periodic acid within the composite material (0.1-10% w/w relative to polymer) to achieve selective oxidation. By carefully controlling this parameter, the system achieves sufficient oxidation of interfering 1,2-diol compounds like 4β,25-dihydroxyvitamin D3 while maintaining stability and selectivity for the target analyte, ensuring reliable and precise extraction.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly reduces processing time, simplifies the extraction process, and lowers costs by selectively removing contaminants like 4β,25-dihydroxyvitamin D3, enabling rapid and cost-effective detection of 1α,25-dihydroxyvitamin D3 using LC-MS/MS analysis.
Implementation Method 1
an oxidizing agent which is a compound capable of selectively oxidizing 1,2-diol compounds
Implementation Method 2
solid phase particles with which the oxidizing agent is mixed
Data Source
AI summary
Provided is a solid phase mixture including an oxidizing agent and/or a salt of the oxidizing agent and solid phase particles. The oxidizing agent is a compound capable of selectively oxidizing 1,2-diol compounds. In addition, a packing material containing the solid phase mixture is provided. Further, a column packed with the packing material is provided.