Solid Phase Multi-Analyte Assay Merging
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Solution Overview
Problem
Current methods for determining the presence and amount of multiple analytes in a sample require separate solid phase components, leading to increased cost, complexity, and time, making them inefficient and prone to operator errors.
Innovation Solution
The use of solid phase analyte compositions with multiple analytes bound to a single solid phase support, allowing for simultaneous, serial, or tandem detection using competitive immunoassays with differentially labeled antibodies.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If separate solid phase components are used for each analyte, then detection of multiple analytes is achieved, but cost, time, and complexity increase
Solution Approach 1:
Multiple different analytes are bound to a single solid phase component, allowing simultaneous detection of multiple analytes in one assay instead of requiring separate components for each analyte
Solution Approach 2:
A single solid phase component serves multiple functions by binding multiple different analytes, enabling the same component to be used for detecting multiple different analytes through competitive immunoassay
2Measurement precision
If separate solid phase components are used for each analyte, then detection of multiple analytes is achieved, but time consumption increases
Solution Approach 1:
Multiple different analytes are bound to a single solid phase component, allowing simultaneous detection of multiple analytes in one assay instead of requiring separate components for each analyte
Solution Approach 2:
The solid phase component with multiple bound analytes can continuously detect multiple analytes in a single continuous assay process, eliminating the need for sequential testing
3Measurement precision
If separate solid phase components are used for each analyte, then detection of multiple analytes is achieved, but cost increases
Solution Approach 1:
Multiple different analytes are bound to a single solid phase component, allowing simultaneous detection of multiple analytes in one assay instead of requiring separate components for each analyte
Solution Approach 2:
A single solid phase component serves multiple functions by binding multiple different analytes, enabling the same component to be used for detecting multiple different analytes through competitive immunoassay
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach reduces the cost, time, and complexity of multi-analyte assays while maintaining high sensitivity and efficiency, enabling rapid detection of multiple analytes in a single assay.
Implementation Method 1
binding between the bound antibody and analyte present in a sample is detected
Implementation Method 2
the solid phase analyte competes with analyte present in a sample for binding to an antibody specific for the analyte
Implementation Method 3
it is labeled, such as radioactively
Implementation Method 4
fluorescently
Implementation Method 5
luminescently
Implementation Method 6
enzymatically (e.g., an enzymatic reaction occurs in the presence of an appropriate substrate, resulting in a color change)
Data Source
AI summary
Compositions and methods for detecting the presence and/or amount of one or more analytes, including analytes such as drugs of abuse, are provided. The compositions include two or more analytes associated with a solid phase, e.g., a particle or a multiwell plate. The compositions and methods also allow the simultaneous, tandem, or serial determination of the presence and/or amount of two or more analytes of interest in a sample.