Solid-State Fermentation Enzyme Formulation for Organic Plant Proteolysis
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Solution Overview
Problem
The high cost of organic plant protease hydrolysate production due to low proteolysis efficiency and the difficulty in meeting organic certification requirements due to the use of non-organic certified substances like strong acids and alkalis in enzymatic hydrolysis processes.
Innovation Solution
A solid-state fermentation enzyme formulation enriched with neutral protease, acidic protease, alkaline protease, glucoamylase, and cellulase, prepared using protease-producing microorganisms such as Aspergillus oryzae and Bacillus sp., combined with a synergistic effect from additional enzymes like papain and bromelain, to enhance proteolysis efficiency and meet organic certification standards.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional enzymatic hydrolysis is used to produce organic plant protease hydrolysate, then the process can produce nitrogen sources, but the proteolysis efficiency is low and production cost is high
Solution Approach 1:
The patent combines multiple protease-producing microorganisms (Aspergillus oryzae, Aspergillus niger, and Bacillus sp.) into a composite enzyme formulation that produces synergistic effects. This merging of different microbial strains creates a more efficient proteolytic system that can achieve over 43% amino nitrogen conversion rate, significantly improving proteolysis efficiency while reducing production costs compared to using single strains or traditional enzymatic methods.
Solution Approach 2:
The invention creates a composite enzyme formulation containing multiple types of proteases (neutral protease, acidic protease, alkaline protease) along with glucoamylase and cellulase. This composite material approach allows the enzyme formulation to tackle different aspects of protein hydrolysis simultaneously, achieving comprehensive and efficient proteolysis of plant proteins while maintaining cost-effectiveness.
2Reliability
If strong acids and alkalis are added to control pH during enzymatic hydrolysis, then the hydrolysis process can be maintained, but the hydrolysate cannot pass organic certification
Solution Approach 1:
The solid-state fermentation process allows the microorganisms to self-regulate the pH environment during fermentation through their metabolic activities. The protease-producing microorganisms create and maintain optimal pH conditions for enzyme activity without requiring external addition of strong acids or alkalis. This self-service approach ensures organic certification compliance while simplifying the manufacturing process by eliminating the need for complex pH control systems.
Solution Approach 2:
The solid-state fermentation system acts as an intermediary that mediates between the need for controlled pH conditions and organic certification requirements. Instead of directly adding chemical pH adjusters, the system uses microorganisms and their metabolic products to naturally buffer and regulate pH, serving as a biological mediator that satisfies both process control and certification needs.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process significantly improves plant proteolysis efficiency, achieving an amino nitrogen conversion rate exceeding 43% and meets EU organic certification requirements by avoiding the use of non-organic certified substances, thereby increasing product value.
Implementation Method 1
enzymatic hydrolysis requires the addition of a large number of biological enzymes
Implementation Method 2
improve proteolysis efficiency, achieving an amino nitrogen conversion rate exceeding 43%
Implementation Method 3
The organic solid-state fermentation enzyme formulation is prepared by solid-state fermentation of protease-producing microorganisms
Implementation Method 4
combined with a synergistic effect from additional enzymes like papain and bromelain, to enhance proteolysis efficiency
Data Source
AI summary
An organic solid-state fermentation enzyme formulation and an organic plant protease hydrolysate, and preparation methods therefor. The organic solid-state fermentation enzyme formulation is prepared by solid-state fermentation of protease-producing microorganisms, wherein the organic solid-state fermentation enzyme formulation comprises neutral protease, acidic protease, alkaline protease, glucoamylase, and cellulase. By means of using the protease-producing microorganisms to perform solid-state fermentation on organic plant raw materials, the organic solid-state fermentation enzyme formulation rich in the acidic protease, the neutral protease, the alkaline protease, the cellulase, and the glucoamylase is obtained, and then a small amount of additional enzyme is added in the organic solid-state fermentation enzyme formulation, thereby improving the plant proteolysis efficiency by means of an enzyme system synergistic complementary effect.