Solid-Support Identifier Sequences for Faster Nucleic Acid Analysis
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Solution Overview
Problem
Existing nucleic acid sequence analysis methods are inefficient and require numerous steps, hindering rapid and accurate determination of target nucleotide sequences.
Innovation Solution
The use of solid supports with single-stranded solid phase nucleic acid comprising identifier and probe sequences, allowing for efficient hybridization and extension of sample nucleic acids, followed by amplification and analysis of the resulting sequences.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional nucleic acid sequence analysis methods are used, then sequence analysis can be performed, but the number of steps required is large and efficiency is low
Solution Approach 1:
The patent combines multiple functional sequences (primer, identifier, and probe sequences) into a single solid support molecule, eliminating the need for separate hybridization and amplification steps. This merging of functions directly reduces the number of operational steps while maintaining sequence analysis capability, thereby improving productivity without sacrificing analytical rigor.
Solution Approach 2:
The solid support is designed with multi-functionality, serving simultaneously as a primer for extension, an identifier for bead recognition, and a probe for target hybridization. This universal design allows a single component to perform multiple roles that traditionally required separate reagents and steps, streamlining the overall process and reducing complexity.
2Measurement precision
If multiple separate steps are used for hybridization and extension, then sequence analysis can be performed, but time consumption increases
Solution Approach 1:
The solid support is pre-prepared with all necessary functional sequences (primer, identifier, and probe) integrated into a single molecule before the analysis begins. This preliminary integration means that during the actual analysis, no additional assembly or sequential addition of components is needed, significantly reducing the time required while ensuring that all necessary functions are present for accurate sequence determination.
Solution Approach 2:
By merging the primer, identifier, and probe sequences into a single solid support molecule, the patent enables simultaneous hybridization and extension in a single reaction step. This eliminates the sequential time requirements of separate hybridization and extension steps, thereby reducing total analysis time while maintaining the precision needed for accurate sequence determination.
3Reliability
If traditional methodologies are used, then sequence analysis can be performed, but the process requires numerous separate operations
Solution Approach 1:
The solid support's multi-functional design integrates primer, identifier, and probe sequences into one component, allowing a single reagent to perform multiple operations. This universality simplifies the operational workflow by reducing the number of separate operations the user must perform, while the integrated design ensures that all necessary functions are present for reliable and accurate sequence analysis.
Solution Approach 2:
The patent merges multiple operational functions into a single solid support molecule, which simultaneously provides hybridization, extension initiation, and identification capabilities. This consolidation reduces the number of separate operations required while maintaining the reliability of sequence analysis, as all critical functions are embedded within the single integrated structure.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach minimizes the number of steps required for nucleic acid sequence analysis, enabling rapid and efficient determination of target sequences, including detection of mutations, haplotyping, and identification of disease markers.
Implementation Method 1
the probe sequence hybridizes to sample nucleic acid when the probe sequence is complementary to a nucleotide sequence in the sample nucleic acid
Data Source
AI summary
Improved solid supports and methods for analyzing target nucleotide sequences are provided herein. Certain improvements are directed to efficiently preparing nucleic acids that comprise nucleotide sequences identical to or substantially identical to one or more target nucleotide sequences, or complement thereof. The prepared nucleic acids include a reference sequence that facilitates sequence analysis. The solid supports and methods provided herein minimize the number of steps required by published sequence analysis methodologies, and thereby offer improved sequence analysis efficiency.