Soluble Beads for Reproducible Standard Solutions
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Solution Overview
Problem
The preparation of standard solutions for spectrometry calibration and diagnostic methods is time-consuming and requires special attention to ensure reproducibility and stability, with existing methods like lyophilization not producing calibrated particles suitable for standard solutions.
Innovation Solution
A process involving soluble beads with a uniform diameter, calibrated to support multiple products grafted onto their surface, which are dissolved in a solvent to create solutions of predetermined concentrations, ensuring complete release of the products without denaturation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If standard solutions are prepared using conventional methods (lyophilization, manual dissolution), then the solutions can be produced, but the process is time-consuming and requires special attention to ensure reproducibility and stability
Solution Approach 1:
The patent applies preliminary action by pre-grafting multiple standardized products onto the surface of soluble beads during manufacturing. Each bead is calibrated to carry precise amounts of multiple products (at least ten different products) in predetermined quantities. This pre-preparation eliminates the need for time-consuming manual weighing and dissolution operations, while ensuring reproducible and stable standard solutions when the beads are dissolved in solvent.
2Productivity
If multiple products are prepared separately and then combined, then each product can be precisely controlled, but the process becomes complex and time-consuming
Solution Approach 1:
The patent merges multiple products onto a single soluble bead support. Each bead is functionalized to graft at least ten different products simultaneously on its surface. When the bead dissolves in solvent, all grafted products are released together, providing a complete multi-product standard solution in one simple dissolution step, thereby eliminating complex multi-step preparation procedures.
Solution Approach 2:
The soluble beads serve as a universal platform that can carry multiple different products (proteins, peptides, enzymes, chemicals) on their surfaces. This multi-functional bead design allows a single bead type to provide standard solutions for multiple analytes, simplifying the overall preparation process while maintaining precise control over each product's concentration.
3Reliability
If products are grafted onto soluble beads, then complete release and preserved activity are achieved, but the grafting process must ensure no denaturation occurs
Solution Approach 1:
The patent employs parameter changes by carefully controlling the grafting conditions (chemical parameters, temperature, pH) to ensure that products are attached to the bead surface without denaturation. The grafting process uses mild conditions that preserve the native structure and activity of proteins, peptides, and enzymes. Subsequent dissolution in appropriate solvents further maintains product stability and complete release.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method allows for the easy and reproducible preparation of standard solutions with preserved enzymatic activity, suitable for spectrometry analysis, enabling efficient calibration and quantification of compounds.
Implementation Method 1
comprises the dissolution of one or more soluble beads in a solvent of the solution on the surface of which said products are grafted
Data Source
Figure 1~2
Figure 3A~3B
AI summary
The present invention relates to the preparing of solutions, in particular for performing analysis methods, in particular by spectrometry, especially for the production of standard solutions that are useful, for example, in the calibration of spectrometry apparatus or for performing diagnostic methods. The invention enables the performing of an easy preparation method for such standard solutions. In particular, the present invention relates to the preparation of a solution of at least one product to be dissolved, comprising the dissolving of one or more soluble balls in the solvent of the solution, to the surface of which said product or products are grafted.