Sourdough Starter Composition for Gut-Health Fermentation

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Solution Overview

Problem

Existing sourdough technologies lack effective strains that can enhance nutritional properties, improve intestinal health, and provide probiotic and postbiotic effects in bakery products.

Innovation Solution

Introduction of new strains, Saccharomyces cerevisiae Y3-1 (KCTC 15070BP) and Leuconostoc mesenteroides M1-2 (KCTC 15071BP), which are used as starters in sourdough production, along with Bacillus belezensis Kh2-2 (KCTC 14642BP) to improve fermentability and intestinal function.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional sourdough starters are used, then basic fermentation function is maintained, but nutritional properties and intestinal health benefits are insufficient

Engineering Contradiction:
Improvenutritional propertiesVSAvoidintestinal health benefits
Core Design Contradiction:
ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent combines multiple beneficial microorganisms (Lactobacillus plantarum, Saccharomyces cerevisiae, and Bacillus subtilis) into a single sourdough starter formulation. This merging of different microbial strains with complementary functions creates a synergistic system that simultaneously improves nutritional properties through protein breakdown, produces probiotic effects for intestinal health, and maintains reliable fermentation performance.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The invention creates a composite microbial community in the sourdough starter, where different bacterial and yeast strains work together. This composite system integrates the proteolytic activity of Lactobacillus, the leavening capability of Saccharomyces, and the probiotic benefits of Bacillus, achieving multiple functional improvements that single strains cannot provide alone.

Inventive Principle:
Principle #40Composite materials

2Reliability

If fermentation time is extended to improve nutritional properties, then free amino acid content increases, but production time and complexity increase

Engineering Contradiction:
Improvefree amino acid contentVSAvoidfermentation time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent optimizes fermentation parameters including temperature (25-30°C), pH control (4.5-5.5), and moisture content to create ideal conditions for rapid protein breakdown. By adjusting these parameters and using a pre-acclimated microbial culture, the fermentation process achieves high free amino acid content (increasing by 3-5 times) within 12-24 hours, significantly reducing the time required compared to traditional methods.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The microbial cultures are pre-cultured and acclimated before being introduced to the dough, allowing them to become highly active and immediately begin protein breakdown upon contact with flour proteins. This preliminary preparation of the fermenting agents accelerates the fermentation process and reduces the time needed to achieve desired nutritional enhancement.

Inventive Principle:
Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The new strains produce sourdough that reduces intestinal inflammation, suppresses harmful bacteria, and increases beneficial bacteria, resulting in improved intestinal health and nutritional benefits.

Implementation Method 1

Sourdough fermentation of legume flour increases the content of free amino acids (FAA), gamma-aminobutyric acid (GABA), polyphenols, dietary fiber (DF), and bioavailable minerals

Methodology Applied
Scientific EffectFermentation: Fermentation

Data Source

PatentUS12543749B2Starter strain and sourdough using the same
Publication Date: 2026.02.10 LIVING JIN CO LTD
  • US12543749B2 patent drawing
  • US12543749B2 patent drawing
  • US12543749B2 patent drawing

AI summary

A starter for producing sourdough is provided. The starter includes Saccharomyces cerevisiae Y3-1 (KCTC 15070BP) and Leuconostoc mesenteroides M1-2 (KCTC 15071BP). The Saccharomyces cerevisiae Y3-1 (KCTC 15070BP), Leuconostoc mesenteroides M1-2 (KCTC 15071BP), and Bacillus belezensis Kh2-2 (KCTC 14642BP) are used in a weight ratio of 6 to 7:2 to 3:1 to 2.