Soy Acid Protein Molecules for Transgenic Crop Trait Enhancement
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Solution Overview
Problem
Current technologies in plant genetics and developmental biology lack effective methods to introduce stable, recombinant DNA constructs that confer enhanced traits such as water use efficiency, cold tolerance, and herbicide tolerance in transgenic plants, with limited success in integrating and expressing these traits consistently across plant populations.
Innovation Solution
The development of recombinant DNA constructs characterized by specific polynucleotide sequences and their cognate proteins, which are stably integrated into plant cell nuclei, expressing proteins with high identity to reference sequences, providing enhanced traits like water use efficiency, cold tolerance, and herbicide tolerance, and methods for selecting and breeding plants with these traits.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If recombinant DNA constructs are introduced into plant cells, then enhanced traits (water use efficiency, cold tolerance, herbicide tolerance) are achieved, but stable integration and consistent expression across plant populations is limited
Solution Approach 1:
The patent applies preliminary action by pre-assembling complete recombinant DNA constructs containing the polynucleotide sequences (SEQ ID NOs: 1-91967) and cognate protein coding regions before introduction into plant cells. This pre-prepared construct approach ensures that all necessary genetic elements are properly configured in advance, facilitating stable integration and consistent expression of enhanced traits across plant populations without requiring complex post-integration modifications
2Productivity
If polynucleotide sequences are introduced to express cognate proteins, then enhanced agronomic traits are produced, but the complexity of verifying stable integration and expression increases
Solution Approach 1:
The patent implements feedback mechanisms by incorporating specific polynucleotide sequences (SEQ ID NOs: 1-91967) that encode cognate proteins with defined amino acid sequences (SEQ ID NOs: 91968-183370). These sequences serve as molecular markers that enable verification of stable integration and consistent expression. The feedback is achieved through the predictable relationship between the introduced polynucleotides and the resulting protein products, allowing researchers to confirm successful transformation and trait expression
Solution Approach 2:
The patent uses the cognate proteins as intermediaries between the introduced polynucleotide sequences and the enhanced agronomic traits. These proteins act as mediators that translate the genetic information into functional phenotypes (water use efficiency, cold tolerance, herbicide tolerance). By identifying and characterizing these intermediary proteins, the patent simplifies the detection and measurement of trait expression, as the presence and activity of these specific proteins can be directly assessed to confirm successful genetic transformation
Data Source
AI summary
This disclosure provides purified nucleic acids and polypeptides. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.