Soybean Variety 01045684 Marker-Assisted Breeding

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, and often result in varied genetic combinations that are difficult to predict.

Innovation Solution

The development of the soybean variety 01045684, which combines improved traits through specific breeding methods including backcrossing and genetic transformation, introducing traits like glyphosate tolerance and resistance to diseases and pests, and utilizing molecular marker profiling for selection and validation.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding techniques are used, then soybean varieties can be developed with desirable traits, but the process is time-consuming and unpredictable

Engineering Contradiction:
Improvepredictability of trait inheritanceVSAvoidbreeding cycle duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces conventional mechanical breeding methods (cross-pollination, selection, and generation advancement) with molecular marker-based identification systems. By using DNA markers linked to desirable traits, breeders can directly identify plants carrying target genes without relying on time-consuming phenotypic selection across multiple generations, thus substituting a biological/mechanical process with a molecular detection system that accelerates the breeding cycle and improves predictability.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Loss of information

If conventional breeding techniques are used, then soybean varieties can be developed, but the genetic combinations are difficult to predict

Engineering Contradiction:
Improvegenetic information trackingVSAvoidprediction accuracy of genetic combinations
Core Design Contradiction:
Loss of informationVSReliability

Solution Approach 1:

The patent implements a feedback mechanism where molecular markers provide direct information about the genetic composition of breeding plants. By detecting specific DNA sequences associated with desirable traits, the system provides real-time feedback on which plants carry the target genetic combinations, allowing breeders to make informed selection decisions rather than relying on unpredictable phenotypic expression or traditional pedigree tracking.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent introduces molecular markers as intermediary elements that mediate between the target genes and the breeder's detection capability. These markers serve as proxies or indicators for the actual desirable traits, allowing indirect detection and tracking of genetic combinations without requiring direct observation of complex genetic interactions or phenotypic expressions.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Productivity

If molecular marker profiling is used for selection and validation, then breeding efficiency is enhanced, but the complexity of the breeding process increases

Engineering Contradiction:
Improvebreeding efficiencyVSAvoidbreeding process complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent extracts the critical genetic information from complex phenotypic observations and concentrates it into specific molecular marker detections. By focusing only on the essential DNA sequences that indicate desirable traits, the system simplifies the selection process despite the introduction of molecular techniques, as breeders no longer need to evaluate multiple phenotypic characteristics across many plants but can directly identify target genotypes through marker presence.

Inventive Principle:
Principle #2Taking out (Extraction)

Data Source

PatentUS9338963B2Soybean variety 01045684
Publication Date: 2016.05.17 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01045684. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01045684. Also provided by the invention are tissue cultures of the soybean variety 01045684 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01045684 with itself or another soybean variety and plants produced by such methods.