Soybean 01045719 Locus Conversion for Trait Stability
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Solution Overview
Problem
Current soybean breeding methods face challenges in developing stable, high-yielding soybean varieties with desirable traits such as disease resistance, herbicide tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, leading to genetic variability and environmental dependence.
Innovation Solution
The development of the soybean variety 01045719, which includes a single locus conversion through genetic transformation, enabling traits like herbicide resistance, disease resistance, and improved nutritional quality, and the use of advanced breeding techniques like backcrossing and marker-assisted selection to stabilize these traits across generations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If conventional breeding techniques are used to develop soybean varieties, then genetic diversity is maintained, but the breeding process becomes time-consuming and unpredictable
Solution Approach 1:
The patent applies parameter changes by using genetic transformation to directly modify specific genetic parameters (introducing transgenes for herbicide resistance, disease resistance, and nutritional quality) rather than relying on conventional breeding. This changes the fundamental parameter of how genetic traits are introduced, moving from gradual selection to direct genetic modification, thereby reducing breeding time while maintaining genetic stability through targeted locus conversion.
2Adaptability or versatility
If conventional breeding techniques are used, then multiple traits can be combined, but the process is unpredictable and leads to genetic variability
Solution Approach 1:
The patent applies local quality by using site-directed recombination to precisely modify specific loci (local regions) of the soybean genome. Instead of relying on random genetic recombination across the entire genome, the technology targets specific locations to introduce desired traits (herbicide resistance, disease resistance, nutritional quality) while maintaining the integrity and stability of the rest of the genome, thereby improving reliability and predictability of trait combinations.
3Loss of time
If genetic transformation is used to introduce traits, then breeding time is reduced, but the complexity of the transformation process increases
Solution Approach 1:
The patent applies mechanics substitution by replacing complex mechanical breeding operations (crossing, selection, and generation advancement) with a biochemical/genetic approach. Site-directed recombination and genetic transformation provide a more direct, controlled mechanism for introducing traits, substituting the mechanical complexity of traditional breeding with a more precise molecular biology-based system that reduces overall process time despite initial transformation complexity.
4Stability of the object's composition
If multiple breeding steps are used to stabilize traits, then trait stability is achieved, but the number of breeding steps increases
Solution Approach 1:
The patent applies preliminary action by performing site-directed recombination and genetic transformation early in the breeding process to directly introduce and stabilize desired traits. Rather than requiring multiple sequential breeding steps to achieve trait stability, the technology establishes the desired genetic configuration upfront through targeted locus conversion, thereby reducing the number of subsequent breeding steps needed while maintaining trait stability.
Data Source
AI summary
The invention relates to the soybean variety designated 01045719. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01045719. Also provided by the invention are tissue cultures of the soybean variety 01045719 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01045719 with itself or another soybean variety and plants produced by such methods.