Soybean Variety 01045768 Genetic Transformation

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, leading to difficulties in consistently producing superior genetic combinations.

Innovation Solution

The development of the soybean variety 01045768, which involves crossing with itself or other plants to produce hybrid seeds and plants, utilizing techniques like backcrossing and genetic transformation to introduce desired traits like herbicide resistance, disease resistance, and improved nutritional quality, while maintaining the morphological and physiological characteristics of the parent variety.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding techniques are used to develop new soybean varieties, then the process is time-consuming and unpredictable, but the methods are simple and do not require complex genetic modification procedures

Engineering Contradiction:
Improvepredictability of desirable trait combinationsVSAvoidtime required for breeding process
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces conventional mechanical breeding techniques with genetic transformation methods. Specifically, it uses Agrobacterium-mediated transformation and particle bombardment to introduce desired traits directly into soybean plants, bypassing the need for lengthy cross-breeding experiments and traditional selection processes.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent employs preliminary action by pre-characterizing parent lines with desirable traits before transformation. The parent soybean plants are selected and characterized for specific traits (disease resistance, drought tolerance, nutritional quality) before genetic transformation, allowing for predictable combination of traits in the final variety without time-consuming trial-and-error breeding.

Inventive Principle:
Principle #10Preliminary action

2Manufacturing precision

If conventional breeding techniques are used, then the breeding process is straightforward, but it produces inconsistent and unpredictable genetic combinations

Engineering Contradiction:
Improveconsistency of desirable trait combinationsVSAvoidcomplexity of breeding methodology
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent replaces the mechanical process of cross-breeding and manual selection with molecular genetic transformation techniques. Using Agrobacterium-mediated transformation and particle bombardment, specific genes can be introduced with precise control, ensuring consistent genetic combinations while simplifying the overall process through molecular rather than mechanical means.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent applies parameter changes by modifying the genetic material itself rather than relying on phenotypic selection. By changing the DNA sequence through genetic transformation, the patent achieves precise control over trait combinations, transforming the breeding process from a statistical guessing game to a controlled molecular modification process.

Inventive Principle:
Principle #35Parameter changes

3Productivity

If genetic transformation techniques are used to introduce desired traits, then the process becomes more efficient and predictable, but it requires complex procedures and specialized equipment

Engineering Contradiction:
Improveefficiency of variety developmentVSAvoidcomplexity of transformation procedures
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent uses an intermediary approach by employing Agrobacterium as a natural vector for gene transfer. This biological intermediary simplifies the transformation process compared to direct physical bombardment, as Agrobacterium naturally transfers DNA into plant cells through a well-understood mechanism, reducing the need for complex equipment while maintaining high efficiency.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent applies self-service by utilizing the plant's own cellular mechanisms for transformation. The Agrobacterium method leverages the plant's natural cell wall permeability and cellular uptake mechanisms, allowing the transformation process to occur through the plant's own biological systems rather than requiring external complex equipment or invasive procedures.

Inventive Principle:
Principle #25Self-service

Data Source

PatentUS9155264B2Soybean variety 01045768
Publication Date: 2015.10.13 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01045768. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01045768. Also provided by the invention are tissue cultures of the soybean variety 01045768 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01045768 with itself or another soybean variety and plants produced by such methods.