Soybean Variety 01045914 Breeding via Segmentation and Feedback

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, leading to significant research costs and variability in results.

Innovation Solution

The development of the soybean variety 01045914, which incorporates a single locus conversion through genetic transformation, conferring traits like herbicide resistance, disease resistance, and improved nutritional quality, and can be propagated using methods like backcrossing and hybridization to produce consistent and superior soybean plants.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional breeding techniques are used to develop soybean varieties, then genetic diversity and trait combinations can be achieved, but the process is time-consuming and unpredictable

Engineering Contradiction:
Improvetrait combination capabilityVSAvoidbreeding cycle duration
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The breeding process is segmented into distinct phases: initial cross-breeding to introduce desired traits, followed by backcrossing to recover the recurrent parent's genome, and final selection. This segmentation allows systematic introduction of specific traits while maintaining control over the breeding timeline and reducing unpredictability.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The recurrent parent is selected and characterized in advance for desired agronomic traits. The breeding program is designed with predetermined goals for trait introduction and recovery. This preliminary planning reduces the time required for trial-and-error breeding cycles and makes the process more predictable.

Inventive Principle:
Principle #10Preliminary action

2Adaptability or versatility

If conventional breeding techniques are used to develop soybean varieties, then natural genetic variation can be utilized, but research costs are significant

Engineering Contradiction:
Improvegenetic variation utilizationVSAvoidresearch cost
Core Design Contradiction:
Adaptability or versatilityVSLoss of energy

Solution Approach 1:

The recurrent parent's desirable genome is copied and recovered through multiple backcrossing generations. This allows the successful combination of introduced traits with the proven performance of the recurrent parent to be replicated and stabilized, reducing the need for repeated expensive breeding programs for similar varieties.

Inventive Principle:
Principle #26Copying

Solution Approach 2:

The breeding program systematically changes genetic parameters through controlled cross-breeding and backcrossing ratios. By adjusting the number of backcross generations and selection criteria, researchers can optimize the balance between introducing new traits and maintaining parent characteristics, thereby reducing unnecessary research expenditures.

Inventive Principle:
Principle #35Parameter changes

3Stability of the object's composition

If multiple backcrossing generations are performed to recover recurrent parent traits, then genetic purity is improved, but the breeding process becomes more complex

Engineering Contradiction:
Improvegenetic purityVSAvoidbreeding process complexity
Core Design Contradiction:
Stability of the object's compositionVSDevice complexity

Solution Approach 1:

Molecular markers and phenotypic assessments are used at each backcross generation to provide feedback on the degree of recurrent parent genome recovery. This feedback allows researchers to determine when sufficient genetic purity has been achieved, preventing unnecessary additional backcrossing steps and simplifying the overall process while maintaining genetic stability.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

Traditional phenotypic selection alone is replaced or supplemented with molecular marker-assisted selection. This substitution enables more accurate and efficient tracking of genome recovery, reducing the manual complexity of monitoring genetic purity across multiple generations while maintaining or improving precision.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS9277714B2Soybean variety 01045914
Publication Date: 2016.03.08 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01045914. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01045914. Also provided by the invention are tissue cultures of the soybean variety 01045914 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01045914 with itself or another soybean variety and plants produced by such methods.