Soybean Variety 01046074 Marker-Assisted Breeding
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Solution Overview
Problem
Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, leading to significant research costs and variability in resulting soybean varieties.
Innovation Solution
The development of the soybean variety 01046074, which combines improved traits through a specific breeding history and genetic transformation, including the introduction of a transgenic gene for glyphosate tolerance, and the use of advanced breeding techniques like backcrossing and marker-assisted selection to introduce desired traits while maintaining the variety's morphological and physiological characteristics.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional breeding techniques are used to develop soybean varieties, then genetic diversity and trait combinations can be achieved, but the breeding process becomes time-consuming and unpredictable
Solution Approach 1:
The patent employs marker-assisted selection to pre-identify plants carrying desired genetic traits before actual phenotypic expression occurs. Molecular markers are used early in the breeding process to screen for specific genes associated with target traits, allowing breeders to select promising individuals without waiting for multi-generation field testing. This preliminary genetic screening accelerates the breeding timeline while maintaining trait accuracy.
Solution Approach 2:
The patent replaces traditional mechanical phenotypic selection methods with molecular-based genetic analysis. Instead of manually evaluating plant traits through observation and measurement across multiple growing seasons, the invention uses DNA marker technology to directly assess genetic composition. This substitution of mechanical/biological evaluation with molecular analysis dramatically reduces breeding time while improving selection precision.
2Ease of manufacture
If conventional breeding methods are employed, then variety development can proceed with existing resources, but research costs and variability in outcomes increase significantly
Solution Approach 1:
The patent implements marker-assisted selection that provides continuous feedback on the genetic composition of breeding populations. Molecular markers serve as reliable indicators that feedback to breeders about the presence and inheritance of desired traits, enabling data-driven selection decisions. This feedback mechanism replaces subjective phenotypic assessment with objective genetic information, reducing outcome variability and improving breeding reliability.
Solution Approach 2:
The patent replaces subjective human judgment in trait evaluation with objective molecular marker analysis. By substituting manual phenotypic assessment with DNA-based genetic testing, the invention eliminates human error and environmental variability that plague conventional breeding. This substitution provides consistent, reproducible results across different breeders and locations, significantly improving outcome reliability.
3Adaptability or versatility
If transgenic genes are introduced for specific traits like glyphosate tolerance, then desired trait expression is achieved, but the complexity of the breeding process increases
Solution Approach 1:
The patent uses molecular markers as intermediary tools to simplify transgenic breeding. Rather than directly tracking complex transgene integration and expression, the invention employs marker genes that are tightly linked to or co-transformed with the transgene of interest. These marker intermediaries provide easily detectable signals (such as antibiotic resistance or herbicide tolerance markers) that indicate successful transgene incorporation, simplifying the selection process for transgenic events.
Solution Approach 2:
The patent performs preliminary selection for transgenic traits using molecular markers early in the breeding process, before complex phenotypic evaluation is needed. By screening for transgene presence and proper integration patterns at the DNA level, the invention filters breeding populations to retain only those individuals with correct transgenic modifications. This preliminary genetic screening prevents the propagation of unwanted transgenic variants and reduces the complexity of later breeding steps.
Data Source
AI summary
The invention relates to the soybean variety designated 01046074. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01046074. Also provided by the invention are tissue cultures of the soybean variety 01046074 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01046074 with itself or another soybean variety and plants produced by such methods.