Soybean Variety 01046137 Genetic Engineering

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, leading to significant research costs and variability in resulting soybean varieties.

Innovation Solution

The development of the soybean variety 01046137, which incorporates specific breeding techniques like backcrossing and genetic transformation to introduce desired traits like herbicide resistance, disease resistance, and improved nutritional quality, while maintaining the morphological and physiological characteristics of the parent variety, and the use of tissue culture methods for regeneration and propagation.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional breeding techniques are used to develop soybean varieties, then genetic diversity and adaptability are maintained, but the breeding process becomes time-consuming and unpredictable

Engineering Contradiction:
Improvegenetic diversityVSAvoidbreeding process duration
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent replaces conventional mechanical breeding methods (cross-pollination, selection, and multi-generation breeding) with genetic engineering techniques. Specifically, it uses Agrobacterium-mediated transformation and direct DNA transfer methods to introduce desired traits directly into the soybean genome, bypassing the need for traditional cross-breeding and selection processes that span multiple generations.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent performs preliminary genetic modification by introducing desired traits directly into the parental line before breeding occurs. This allows the parent plants to already possess the desired characteristics (such as herbicide resistance or improved nutritional content) before crossing, eliminating the need for time-consuming selection processes in subsequent generations.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If conventional breeding techniques are used to develop soybean varieties, then natural genetic variation is preserved, but research costs increase due to unpredictability

Engineering Contradiction:
Improvegenetic variationVSAvoidresearch costs
Core Design Contradiction:
Stability of the object's compositionVSLoss of energy

Solution Approach 1:

The patent replaces unpredictable conventional breeding with precise genetic engineering methods. By using molecular markers and targeted gene transfer, researchers can predictably introduce specific traits without the variability inherent in traditional cross-breeding, thereby reducing research costs associated with screening and selecting through multiple generations.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent employs molecular marker-assisted selection and genetic screening methods to monitor and verify the introduction of desired traits. This feedback mechanism allows researchers to confirm successful genetic modification early in the process, eliminating the need for costly and time-consuming field trials across multiple generations to verify trait inheritance.

Inventive Principle:
Principle #23Feedback

3Reliability

If multiple breeding generations are pursued to stabilize traits, then trait consistency is achieved, but the breeding cycle extends significantly

Engineering Contradiction:
Improvetrait consistencyVSAvoidbreeding cycle speed
Core Design Contradiction:
ReliabilityVSProductivity

Solution Approach 1:

The patent achieves trait stabilization in a single generation by directly introducing the desired genetic trait into the parental line through genetic engineering. This preliminary action ensures that the parent plants already possess the stable, consistent trait before breeding, eliminating the need for multiple generations of selection and stabilization that traditionally extend the breeding cycle.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent replaces the multi-generation selection process with direct genetic transformation methods. By using Agrobacterium-mediated transformation or direct DNA transfer, the desired trait is permanently and stably integrated into the genome in a single step, achieving trait consistency without the time-consuming iterative process of conventional breeding.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

4Ease of manufacture

If traditional breeding methods are used to introduce new traits, then genetic integrity is maintained, but the precision of trait introduction is reduced

Engineering Contradiction:
Improvegenetic integrityVSAvoidtrait introduction precision
Core Design Contradiction:
Ease of manufactureVSManufacturing precision

Solution Approach 1:

The patent replaces imprecise conventional breeding with precise genetic engineering techniques. By using site-specific recombination systems and molecular markers, researchers can introduce desired traits at specific locations in the genome with high precision, while maintaining the overall genetic integrity of the parental line through controlled transformation methods.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS9029650B2Soybean variety 01046137
Publication Date: 2015.05.12 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01046137. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01046137. Also provided by the invention are tissue cultures of the soybean variety 01046137 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01046137 with itself or another soybean variety and plants produced by such methods.