Soybean Variety 01046439 Genetic Transformation for Trait Stability

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, leading to significant research costs and variability in results.

Innovation Solution

The development of the soybean variety 01046439, which incorporates a single locus conversion through genetic transformation, conferring traits like herbicide resistance, disease resistance, and improved nutritional quality, and can be propagated using methods like backcrossing and hybridization to produce consistent and superior soybean plants.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional breeding techniques are used to develop soybean varieties, then genetic diversity and trait combinations can be achieved, but the process is time-consuming and unpredictable

Engineering Contradiction:
Improvetrait combination capabilityVSAvoidbreeding cycle duration
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent replaces conventional mechanical breeding methods (cross-pollination, selection, and generation advancement) with genetic transformation technology. This allows direct introduction of desired traits through DNA manipulation rather than relying on gradual genetic recombination over multiple generations, thereby dramatically reducing the time required while maintaining the ability to achieve specific trait combinations

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent employs preliminary genetic modification to introduce desired traits directly into the soybean genome before field testing. By pre-establishing the genetic basis for disease resistance, herbicide tolerance, and improved nutritional quality through transformation, the breeding process skips the lengthy stages of traditional cross-breeding and selection, achieving rapid development of varieties with predetermined trait combinations

Inventive Principle:
Principle #10Preliminary action

2Ease of manufacture

If conventional breeding techniques are used, then variety development can proceed with existing methods, but results show significant variability and unpredictability

Engineering Contradiction:
Improvebreeding process simplicityVSAvoidtrait expression consistency
Core Design Contradiction:
Ease of manufactureVSReliability

Solution Approach 1:

The patent changes the fundamental parameter of trait introduction from probabilistic genetic recombination to deterministic genetic transformation. By using recombinant DNA technology to directly insert specific genes with known functions into the soybean genome, the process achieves predictable and consistent trait expression across generations, eliminating the variability inherent in conventional breeding while maintaining operational feasibility through established transformation protocols

Inventive Principle:
Principle #35Parameter changes

3Manufacturing precision

If multiple breeding cycles are conducted to achieve desirable traits, then trait improvement can be achieved, but research costs increase significantly

Engineering Contradiction:
Improvetrait selection accuracyVSAvoidresearch cost
Core Design Contradiction:
Manufacturing precisionVSLoss of energy

Solution Approach 1:

The patent replaces iterative breeding cycles with a single-step genetic transformation process. Instead of conducting multiple rounds of crossing, selection, and backcrossing to achieve desired trait accuracy, the technology directly introduces targeted genes through transformation, achieving the same level of trait precision in one operation and thereby dramatically reducing the cumulative research costs associated with prolonged breeding programs

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

4Productivity

If conventional breeding is used to achieve high yield and stability, then variety improvement can occur, but the process lacks precision in trait incorporation

Engineering Contradiction:
Improvesoybean yieldVSAvoidtrait incorporation precision
Core Design Contradiction:
ProductivityVSManufacturing precision

Solution Approach 1:

The patent transforms the precision parameter by shifting from indirect trait selection based on phenotypic observation to direct genetic modification with known molecular sequences. This allows exact incorporation of specific genes responsible for yield and stability traits, ensuring precise trait introduction while maintaining the productivity goals through targeted selection of high-yielding transformation events

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS9338975B2Soybean variety 01046439
Publication Date: 2016.05.17 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01046439. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01046439. Also provided by the invention are tissue cultures of the soybean variety 01046439 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01046439 with itself or another soybean variety and plants produced by such methods.