Soybean Variety 01046965 Genetic Engineering for Trait Stability

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, with limited control over genetic combinations and environmental factors.

Innovation Solution

The development of the soybean variety 01046965, which involves crossing specific parental lines, using techniques like backcrossing and genetic transformation to introduce desired traits like herbicide resistance, disease resistance, and improved nutritional quality, while maintaining the morphological and physiological characteristics of the parent variety.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding techniques are used to develop soybean varieties, then genetic diversity and trait combination are achieved, but the breeding process is time-consuming and unpredictable

Engineering Contradiction:
Improvepredictability of breeding outcomesVSAvoidbreeding development time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces conventional mechanical breeding methods (crossing, selection, and recombination through physical means) with genetic engineering techniques. Specifically, it uses recombinant DNA technology to directly introduce desired traits into the soybean genome, bypassing the time-consuming process of conventional breeding while achieving predictable trait expression. This substitution of mechanical breeding with molecular biology methods resolves the contradiction between reliability and time loss.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Manufacturing precision

If conventional breeding methods are employed, then natural genetic recombination occurs, but control over specific genetic combinations is limited

Engineering Contradiction:
Improvecontrol over genetic combinationsVSAvoidbreeding program complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent segments the breeding process into distinct molecular steps: isolation of specific genes, cloning into vectors, transformation into soybean cells, and selection of transgenic events. This segmentation allows precise control over which genetic combinations are introduced, replacing the uncontrolled nature of conventional breeding with a stepwise, precisely controlled molecular approach, thereby improving manufacturing precision while managing complexity through systematic procedures.

Inventive Principle:
Principle #1Segmentation

3Adaptability or versatility

If multiple breeding objectives are pursued simultaneously, then comprehensive improvement is achieved, but the breeding program becomes more complex and difficult to manage

Engineering Contradiction:
Improvenumber of traits improvedVSAvoidbreeding program management
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent merges multiple breeding objectives into a single integrated genetic engineering platform. By using universal transformation methods and selectable markers, it can simultaneously introduce multiple desired traits (such as herbicide resistance, disease resistance, and improved yield) into a single soybean variety through one transformation event, rather than requiring separate breeding programs for each trait, thus improving adaptability while reducing program complexity.

Inventive Principle:
Principle #5Merging (Combining)

Data Source

PatentUS9107372B2Soybean variety 01046965
Publication Date: 2015.08.18 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01046965. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01046965. Also provided by the invention are tissue cultures of the soybean variety 01046965 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01046965 with itself or another soybean variety and plants produced by such methods.