Soybean Variety 01050478 Trait Stacking via Genetic Transformation

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding soybean varieties that combine desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, while also being agronomically sound and adaptable to various environmental conditions.

Innovation Solution

The development of the soybean variety 01050478, which involves crossing specific parental lines and using genetic transformation to introduce desirable traits like herbicide resistance, disease resistance, and improved nutritional quality, while maintaining the morphological and physiological characteristics of the original variety through backcrossing and tissue culture techniques.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional breeding methods are used to develop soybean varieties, then genetic diversity and adaptability are maintained, but breeding time and complexity increase significantly

Engineering Contradiction:
ImproveadaptabilityVSAvoidbreeding time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-introducing multiple desirable traits (herbicide resistance, disease resistance, improved nutritional quality) through genetic transformation before variety development begins. This allows breeders to start with genetically improved parental lines rather than developing traits during the breeding process, significantly reducing the time required while maintaining adaptability through careful selection of parental germplasm

Inventive Principle:
Principle #10Preliminary action

2Reliability

If multiple desirable traits are combined in a single variety, then overall performance and resistance improve, but breeding complexity and difficulty increase

Engineering Contradiction:
Improvedisease resistanceVSAvoidbreeding complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent merges multiple desirable traits (herbicide resistance, disease resistance, improved nutritional quality) into single parental varieties through genetic transformation before crossing. This combining approach allows the accumulation of multiple traits in one variety without the complexity of traditional multi-generational selection, as all traits are introduced simultaneously through transformation rather than sequentially through breeding

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The patent uses genetically transformed parental lines as intermediaries that carry pre-introduced desirable traits. These intermediate varieties serve as ready-made sources of multiple traits (herbicide resistance, disease resistance, nutritional quality) that can be combined through simple crossing, avoiding the complexity of directly breeding multiple traits from scratch

Inventive Principle:
Principle #24Intermediary (Mediator)

3Manufacturing precision

If genetic transformation is used to introduce desirable traits, then breeding time is reduced and trait precision is improved, but regulatory and safety considerations increase

Engineering Contradiction:
Improvetrait precisionVSAvoidregulatory complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent applies local quality by introducing specific desirable traits (herbicide resistance, disease resistance, nutritional quality) at precise locations in the genome through targeted genetic transformation. This allows exact control over which traits are introduced and where they are integrated, achieving high trait precision while using well-characterized transformation protocols that have established regulatory pathways

Inventive Principle:
Principle #3Local quality

Data Source

PatentUS9155276B2Soybean variety 01050478
Publication Date: 2015.10.13 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01050478. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01050478. Also provided by the invention are tissue cultures of the soybean variety 01050478 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01050478 with itself or another soybean variety and plants produced by such methods.