Soybean Variety 01050487 Breeding via Marker-Assisted Selection

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, leading to the need for innovative approaches to combine desirable traits effectively.

Innovation Solution

The development of the soybean variety 01050487, which involves crossing and selection methods including backcrossing, genetic transformation, and marker-assisted selection to introduce specific traits like herbicide resistance, disease resistance, and improved nutritional quality, while maintaining the overall agronomic characteristics of the soybean variety.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding techniques are used to develop soybean varieties, then the breeding process can be performed with traditional methods, but the process is time-consuming and unpredictable

Engineering Contradiction:
Improvepredictability of breeding outcomeVSAvoidbreeding development time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces conventional mechanical breeding methods (manual crossing, selection, and breeding) with molecular biology techniques including DNA extraction, PCR amplification, and genetic marker analysis. This substitution enables precise identification and selection of desirable traits at the molecular level, dramatically reducing breeding time and improving predictability of outcomes.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent introduces genetic markers as intermediaries to identify and track desirable traits during breeding. These markers serve as proxies for complex traits, allowing breeders to select plants with desired characteristics more efficiently and predictably, without waiting for phenotypic expression.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Adaptability or versatility

If multiple desirable traits are combined in a single variety, then the soybean variety achieves superior performance, but the complexity of the breeding program increases

Engineering Contradiction:
Improvecombination of desirable traitsVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent segments the breeding program into distinct molecular steps: DNA extraction, specific gene/marker identification, trait-by-trait analysis, and sequential selection. This segmentation allows complex multi-trait breeding to be managed through standardized, repeatable procedures for each trait, reducing overall program complexity.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent changes the parameters of trait selection from phenotypic observation to molecular characterization. By using DNA-based markers and genetic analysis, the breeding program can simultaneously track multiple traits through biochemical parameters rather than complex phenotypic monitoring, simplifying the breeding process.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS9220221B2Soybean variety 01050487
Publication Date: 2015.12.29 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01050487. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01050487. Also provided by the invention are tissue cultures of the soybean variety 01050487 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01050487 with itself or another soybean variety and plants produced by such methods.