Soybean Variety 01050596 Marker-Assisted Breeding

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding soybean varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, and often result in varied genetic combinations.

Innovation Solution

The development of the soybean variety 01050596, which includes specific genetic traits like herbicide resistance and improved fatty acid composition, achieved through a combination of traditional breeding and genetic transformation, allowing for the introduction of desirable genes and traits while maintaining the overall morphological and physiological characteristics of the soybean variety.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding techniques are used to develop soybean varieties, then genetic diversity is maintained, but the breeding process is time-consuming and unpredictable

Engineering Contradiction:
Improvepredictability of breeding outcomeVSAvoidbreeding time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent uses molecular markers as intermediaries to bridge the gap between conventional breeding and trait selection. These markers serve as reliable indicators that allow breeders to predict and select for desired traits (such as disease resistance, herbicide tolerance, and fatty acid composition) without waiting for the traits to manifest phenotypically, thereby accelerating the breeding process while maintaining reliability

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent replaces the traditional mechanical/phenotypic selection system with a molecular/genotypic selection system. Instead of visually assessing and selecting plants based on observable traits after they mature, the invention uses DNA-based molecular markers to identify and select plants with desired genetic characteristics at the seedling stage or even earlier, substituting the slow mechanical breeding process with a faster molecular detection approach

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Adaptability or versatility

If multiple desirable traits are combined in a single variety, then agronomic quality and nutritional value improve, but the genetic complexity increases

Engineering Contradiction:
Improvecombination of desirable traitsVSAvoidgenetic complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent segments the complex task of combining multiple desirable traits into manageable components by using specific molecular markers for each trait. Each marker corresponds to a specific gene or genetic region associated with a particular trait (e.g., Rps3a for Phytophthora resistance, MON89788 for glyphosate tolerance, and specific markers for fatty acid composition). This segmentation allows breeders to independently track and combine different traits through marker-assisted selection, simplifying the management of genetic complexity while achieving versatile multi-trait varieties

Inventive Principle:
Principle #1Segmentation

3Manufacturing precision

If genetic transformation is used to introduce specific traits, then breeding precision improves, but the process becomes more complex

Engineering Contradiction:
Improveprecision of trait introductionVSAvoidbreeding process complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent uses molecular markers as intermediaries to verify and confirm the successful introduction of transgenic traits. After genetic transformation, the markers serve as reliable indicators to identify plants that have successfully incorporated the desired genes (such as MON89788 for glyphosate tolerance or MON87708 for dicamba tolerance). This intermediary system ensures precision in trait introduction by providing molecular confirmation of gene presence and expression, while the standardized marker protocols actually simplify the overall process by providing clear selection criteria

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS9901055B2Soybean variety 01050596
Publication Date: 2018.02.27 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01050596. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01050596. Also provided by the invention are tissue cultures of the soybean variety 01050596 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01050596 with itself or another soybean variety and plants produced by such methods.