Soybean Variety 01050668 Breeding via Segmentation and Markers

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, leading to difficulties in consistently producing superior genetic combinations.

Innovation Solution

The development of the soybean variety 01050668, which includes a single locus conversion that can confer traits like herbicide resistance, disease resistance, and improved nutritional quality, through genetic transformation and backcrossing techniques, allowing for precise introduction of desired genetic loci while maintaining the morphological and physiological characteristics of the original variety.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding techniques are used to develop soybean varieties with desirable traits, then genetic diversity can be maintained, but the breeding process becomes time-consuming and unpredictable

Engineering Contradiction:
Improvepredictability of breeding outcomesVSAvoidbreeding development time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The breeding process is segmented into distinct phases: selecting a recurrent parent with desirable traits, identifying a donor parent with the specific trait of interest, performing controlled crossbreeding, and systematically backcrossing to recover the recurrent parent's genome while retaining the desired trait. This segmentation allows for predictable outcomes by controlling each phase independently.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Preliminary selection of parental lines is performed based on their genetic characteristics and desired traits. The recurrent parent is pre-selected for superior agronomic performance, and the donor parent is pre-selected for the specific trait to be transferred. This preliminary action ensures that the breeding program starts with optimized genetic combinations, reducing the time needed for subsequent selection cycles.

Inventive Principle:
Principle #10Preliminary action

2Manufacturing precision

If multiple breeding cycles are performed to combine desirable traits, then genetic combinations can be improved, but the complexity of the breeding program increases

Engineering Contradiction:
Improveprecision of trait combinationVSAvoidbreeding program complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

Molecular marker-assisted selection provides continuous feedback during the backcrossing process. DNA markers linked to the desired trait are used to track the presence of the target gene in each generation, allowing breeders to make informed decisions about which plants to advance. This feedback mechanism ensures precise trait combination without requiring complex manual tracking of multiple genetic loci.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

Molecular markers serve as intermediaries between the visible phenotype and the underlying genotype. Instead of directly observing and selecting for complex trait combinations, breeders use molecular markers as mediators to indirectly select for the desired genetic combinations. This simplifies the breeding program by replacing complex phenotypic evaluation with straightforward molecular analysis.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Productivity

If traditional breeding methods are used to introduce new traits, then genetic stability can be maintained, but the efficiency of trait introduction decreases

Engineering Contradiction:
Improveefficiency of trait introductionVSAvoidgenetic stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The traditional mechanical process of repeated phenotypic selection and visual evaluation is replaced with molecular marker-based genotypic selection. This substitution allows for rapid identification of plants carrying the desired trait combination, dramatically increasing the efficiency of trait introduction while maintaining genetic stability through precise selection. The molecular markers provide an accurate, non-destructive method to track genetic composition through multiple breeding cycles.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS9532544B2Soybean variety 01050668
Publication Date: 2017.01.03 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01050668. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01050668. Also provided by the invention are tissue cultures of the soybean variety 01050668 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01050668 with itself or another soybean variety and plants produced by such methods.