Soybean Variety 01050728 Molecular Marker Selection

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, leading to genetic variability and environmental dependence.

Innovation Solution

The development of the soybean variety 01050728, which includes a single locus conversion that can confer traits like herbicide resistance, disease resistance, and improved nutritional quality, achieved through genetic transformation and backcrossing techniques, allowing for precise trait introduction while maintaining the variety's morphological and physiological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding techniques are used to develop soybean varieties with desirable traits, then the breeding process is time-consuming and unpredictable, but the resulting varieties exhibit genetic variability and environmental dependence

Engineering Contradiction:
Improvebreeding outcome predictabilityVSAvoidbreeding cycle duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent applies parameter changes by transitioning from conventional phenotypic selection to molecular marker-assisted selection, changing the detection parameter from physical traits to DNA markers. This enables earlier and more accurate identification of desired traits, reducing breeding time while improving predictability. The use of specific molecular markers (e.g., for disease resistance genes like Rps1c, Rps3, Rps4) allows precise tracking of genetic inheritance patterns.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent replaces the mechanical/conventional breeding system with a molecular biology-based system. Instead of relying on observing and selecting plants based on physical characteristics, the invention uses DNA markers and molecular techniques to identify and select for desired traits. This substitution of detection and selection mechanisms dramatically improves both speed and reliability of breeding outcomes.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Reliability

If conventional breeding techniques are used, then the process relies on phenotypic selection, but this leads to genetic variability and environmental dependence

Engineering Contradiction:
Improvetrait consistencyVSAvoidbreeding program complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent replaces phenotypic selection with molecular marker-based selection. By using DNA markers that directly detect genetic traits, the system eliminates the variability introduced by environmental factors affecting phenotypic expression. The molecular markers provide a direct readout of genetic composition, ensuring trait consistency regardless of environmental conditions.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent introduces molecular markers as intermediaries between the genetic material and the selection process. These markers serve as detectable proxies for desired traits, allowing breeders to select based on genetic composition rather than phenotypic expression. This intermediary approach decouples selection from environmental influences, improving trait consistency.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Manufacturing precision

If single locus conversion is used to introduce specific traits, then trait introduction is precise, but the process requires genetic transformation and backcrossing

Engineering Contradiction:
Improvetrait introduction precisionVSAvoidgenetic modification process complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent applies segmentation by focusing on introducing and tracking individual genes or loci of interest rather than dealing with the entire genome. By using specific molecular markers for individual traits (such as Rps1c for Phytophthora resistance, Rps3 for brown stem rot resistance, or herbicide resistance genes), the process breaks down complex trait introduction into manageable, discrete units that can be precisely controlled and tracked through backcrossing.

Inventive Principle:
Principle #1Segmentation

Data Source

PatentUS9510546B1Soybean variety 01050728
Publication Date: 2016.12.06 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01050728. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01050728. Also provided by the invention are tissue cultures of the soybean variety 01050728 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01050728 with itself or another soybean variety and plants produced by such methods.