Soybean Variety 01050761 Genetic Transformation Breeding

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, and often result in varied genetic combinations.

Innovation Solution

The development of the soybean variety 01050761, which includes a single locus conversion that can confer traits like herbicide resistance, disease resistance, and improved nutritional quality, achieved through genetic transformation and backcrossing techniques, allowing for precise introduction of desired genes while maintaining the morphological and physiological characteristics of the original variety.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding techniques are used to develop soybean varieties with desirable traits, then the breeding process is time-consuming and unpredictable, but the resulting varieties often have varied genetic combinations that are difficult to control

Engineering Contradiction:
Improvepredictability of breeding outcomesVSAvoidbreeding cycle duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces conventional mechanical/conventional breeding techniques with genetic transformation methods. Specifically, it uses Agrobacterium-mediated transformation and particle bombardment to introduce desired genes directly into soybean plants, bypassing the need for lengthy cross-breeding cycles and selection processes. This substitution of breeding mechanisms enables precise control over genetic modifications while significantly reducing the time required to develop varieties with specific traits.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Manufacturing precision

If conventional breeding techniques are used to combine desirable traits from parental germplasm, then the process is complex and time-consuming, but the resulting genetic combinations are varied and hard to control

Engineering Contradiction:
Improvecontrol over genetic combinationsVSAvoidbreeding program complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent extracts and isolates specific desirable genes or genetic markers from parental germplasm and introduces them directly into the target soybean variety through genetic transformation. This extraction of specific genetic elements allows for precise control over which traits are combined, eliminating the need to sort through complex genetic combinations that arise in conventional breeding. The process selectively takes out only the desired genetic information and inserts it into the appropriate genomic location.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent utilizes genetic markers and molecular markers to track and select for specific genetic combinations. By changing the approach from phenotypic selection to genotypic selection based on molecular parameters, the breeding process achieves precise control over genetic combinations. The use of marker-assisted selection allows breeders to monitor and control the genetic makeup of plants at the DNA level, enabling accurate prediction and control of trait inheritance.

Inventive Principle:
Principle #35Parameter changes

3Productivity

If conventional breeding techniques are used to develop high-yielding varieties with multiple desirable traits, then the process requires multiple breeding steps and is unpredictable, but the resulting varieties may lack stable performance

Engineering Contradiction:
Improveseed yieldVSAvoidstability of variety performance
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent performs preliminary genetic modifications and trait introductions before the actual breeding process. By pre-introducing desirable genes through genetic transformation and using molecular markers to identify and select for these traits in early generations, the breeding program establishes a stable genetic foundation before field testing and variety release. This preliminary action ensures that the variety achieves both high productivity and stable performance, as the genetic modifications are established and stabilized before the variety is deployed.

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS9585343B2Soybean variety 01050761
Publication Date: 2017.03.07 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01050761. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01050761. Also provided by the invention are tissue cultures of the soybean variety 01050761 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01050761 with itself or another soybean variety and plants produced by such methods.