Soybean Variety 01051074 Marker-Assisted Breeding

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, and often result in varieties that are difficult to replicate.

Innovation Solution

The development of the soybean variety 01051074, which incorporates specific genetic traits through crossing and backcrossing, including transgenic genes for herbicide resistance and tolerance to environmental stresses, along with advanced breeding techniques like genetic marker-assisted selection, to produce consistent and superior soybean plants.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding techniques are used, then soybean varieties can be developed with desirable traits, but the process is time-consuming and unpredictable

Engineering Contradiction:
Improvepredictability of breeding outcomeVSAvoidbreeding development time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces conventional mechanical breeding methods (cross-pollination, selection, and generation advancement) with molecular biology techniques including DNA extraction, PCR amplification of specific loci, and genetic marker analysis. This substitution enables direct genetic assessment rather than relying on phenotypic selection over multiple generations, dramatically reducing both time and increasing predictability of breeding outcomes.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent uses DNA copying and amplification techniques (PCR) to replicate specific genetic sequences from parental plants. By copying and analyzing the genetic markers at targeted loci, breeders can predict offspring traits before actual crossing occurs, enabling selection of parent combinations that will reliably produce desired traits without waiting for phenotypic expression.

Inventive Principle:
Principle #26Copying

2Stability of the object's composition

If conventional breeding techniques are used, then soybean varieties can be developed, but the results are difficult to replicate

Engineering Contradiction:
Improvevariety consistencyVSAvoidbreeding efficiency
Core Design Contradiction:
Stability of the object's compositionVSProductivity

Solution Approach 1:

The patent replaces phenotypic-based selection with genotypic-based selection using DNA marker analysis. By directly analyzing the genetic composition at specific loci, the method ensures that selected plants possess the exact genetic markers associated with desired traits, guaranteeing variety consistency and enabling precise replication across different breeding programs and environments.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent implements a feedback mechanism where DNA marker analysis results from parental plants are used to predict and select optimal crossing combinations. This feedback loop allows breeders to verify genetic composition before crossing, ensuring that only plants with the desired genetic markers are used as parents, thereby maintaining variety stability and improving breeding efficiency.

Inventive Principle:
Principle #23Feedback

3Reliability

If transgenic genes are introduced for herbicide resistance, then crop protection is improved, but genetic complexity increases

Engineering Contradiction:
Improveherbicide resistanceVSAvoidgenetic structure
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent extracts and isolates specific DNA markers associated with herbicide resistance traits from complex genetic backgrounds. By focusing analysis on discrete, identifiable loci rather than the entire genome, the method simplifies the management of transgenic complexity while maintaining reliable herbicide resistance selection through marker-assisted breeding.

Inventive Principle:
Principle #2Taking out (Extraction)

Data Source

PatentUS9974268B2Soybean variety 01051074
Publication Date: 2018.05.22 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01051074. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01051074. Also provided by the invention are tissue cultures of the soybean variety 01051074 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01051074 with itself or another soybean variety and plants produced by such methods.