Soybean Variety 01052178 Genetic Transformation

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, leading to significant research costs and variability in results.

Innovation Solution

The development of the soybean variety 01052178, which incorporates a single locus conversion, including transgenic genes for herbicide resistance and other desirable traits, through genetic transformation, allowing for the production of seeds and plants with consistent morphological and physiological characteristics, and the use of advanced breeding methods like backcrossing and marker-assisted selection to enhance specific traits.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding techniques are used to develop soybean varieties, then genetic diversity can be maintained, but the breeding process becomes time-consuming and unpredictable with significant variability in results

Engineering Contradiction:
Improvepredictability of breeding outcomesVSAvoidbreeding cycle duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces conventional mechanical breeding methods (cross-pollination, selection, and generation advancement) with genetic transformation technology. This involves directly introducing specific genes into the soybean genome using Agrobacterium-mediated transformation or particle bombardment, thereby eliminating the need for multi-generation breeding cycles and achieving predictable trait introduction in a single step.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent changes the fundamental parameter of trait introduction from gradual genetic recombination over multiple generations to direct genetic modification. By altering the method of gene introduction from conventional recombination to targeted transformation, the breeding process achieves both time efficiency and predictability while maintaining genetic diversity through careful selection of transformation methods and protocols.

Inventive Principle:
Principle #35Parameter changes

2Productivity

If conventional breeding techniques are used, then natural genetic variation is preserved, but research and development costs increase due to extended breeding cycles

Engineering Contradiction:
Improvebreeding efficiencyVSAvoidresearch and development costs
Core Design Contradiction:
ProductivityVSLoss of energy

Solution Approach 1:

The patent substitutes costly, multi-year conventional breeding programs with efficient genetic transformation protocols. By directly introducing desired traits through transformation rather than pursuing traits through multiple generations of crossing and selection, the R&D timeline is compressed from years to months, dramatically improving breeding efficiency and reducing associated research costs.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent performs preliminary genetic modification in controlled laboratory conditions before field testing. By pre-introducing and validating target genes in transformation experiments, the patent eliminates the need for extensive preliminary breeding work, thereby reducing overall R&D costs and accelerating the path from concept to commercial variety.

Inventive Principle:
Principle #10Preliminary action

3Stability of the object's composition

If multiple breeding cycles are performed to introduce desirable traits, then trait stability can be achieved, but the process becomes complex and resource-intensive

Engineering Contradiction:
Improvetrait stabilityVSAvoidbreeding program complexity
Core Design Contradiction:
Stability of the object's compositionVSDevice complexity

Solution Approach 1:

The patent replaces complex multi-cycle breeding programs with a single genetic transformation event. By directly introducing stable, integrated transgenes that are inherited according to Mendelian principles, the patent achieves trait stability without requiring repeated crossing, selection, and backcrossing cycles, thereby simplifying the breeding program structure and reducing operational complexity.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent changes the stability achievement mechanism from gradual fixation of traits over multiple generations to immediate stable integration of transformation events. By using well-established transformation protocols that ensure proper gene integration and expression, the patent achieves consistent, stable trait inheritance in the first generation, eliminating the need for complex multi-cycle stabilization programs.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS9510548B1Soybean variety 01052178
Publication Date: 2016.12.06 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01052178. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01052178. Also provided by the invention are tissue cultures of the soybean variety 01052178 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01052178 with itself or another soybean variety and plants produced by such methods.