Soybean Variety 01059966 Breeding Using Marker-Assisted Selection

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, with limited control over genetic combinations and environmental factors.

Innovation Solution

The development of the soybean variety 01059966, which involves a specific breeding history and genetic transformation to introduce desirable traits like herbicide resistance, disease resistance, and improved nutritional quality, using techniques such as backcrossing and genetic marker-assisted selection, allowing for precise introduction of desired genes while maintaining the overall morphological and physiological characteristics of the soybean variety.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional breeding techniques are used to develop soybean varieties, then genetic diversity and adaptability are improved, but breeding time and unpredictability increase

Engineering Contradiction:
Improvegenetic diversityVSAvoidbreeding time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-selecting parental lines with desired traits (disease resistance, yield potential, nutritional quality) before crossing. The breeding program establishes clear selection criteria and pre-characterizes parents using molecular markers to predict progeny performance, reducing the time needed for field testing and selection in subsequent generations.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent implements feedback through molecular marker-assisted selection, where DNA markers are used to track inheritance of desired traits across generations. This provides real-time genetic information feedback that guides selection decisions, allowing breeders to identify promising progeny earlier and with greater accuracy than traditional phenotypic selection alone.

Inventive Principle:
Principle #23Feedback

2Adaptability or versatility

If conventional breeding techniques are used, then natural genetic recombination occurs, but control over genetic combinations is limited

Engineering Contradiction:
Improvegenetic recombinationVSAvoidcontrol over genetic combinations
Core Design Contradiction:
Adaptability or versatilityVSManufacturing precision

Solution Approach 1:

Molecular marker feedback enables precise tracking of specific genetic combinations through generations. Breeders can identify progeny with desired allele combinations at the DNA level, providing control over which parental traits are successfully combined, rather than relying solely on random recombination and later phenotypic selection.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent replaces traditional mechanical/phenotypic selection methods with molecular marker-based genetic analysis. Instead of waiting for plants to express traits physically and then selecting based on observation, the system uses DNA markers to detect and select for desired genetic combinations at the molecular level, providing greater precision and earlier selection.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Adaptability or versatility

If traditional breeding methods are used, then environmental factors are naturally selected, but predictability of breeding outcomes decreases

Engineering Contradiction:
Improveenvironmental adaptationVSAvoidpredictability of outcomes
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

Molecular marker feedback provides reliable genetic information that is independent of environmental conditions. By selecting based on DNA markers rather than phenotypic expression alone, breeders can predict outcomes with greater reliability, as genetic presence is confirmed at the molecular level regardless of environmental variability during testing.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent applies preliminary action by pre-characterizing parental lines with molecular markers for disease resistance and other traits before crossing. This preliminary genetic characterization allows breeders to predict progeny performance with greater reliability, reducing the uncertainty associated with environmental interactions that affect phenotypic expression in traditional breeding.

Inventive Principle:
Principle #10Preliminary action

4Adaptability or versatility

If multiple desirable traits are combined in soybean varieties, then agronomic quality improves, but breeding complexity increases

Engineering Contradiction:
Improveagronomic qualityVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by breaking down the complex task of combining multiple traits into manageable components. Each parental line is selected for specific trait combinations (e.g., one parent for disease resistance, another for yield), and molecular markers are used to track inheritance of each trait separately, making the overall breeding program more manageable and systematic.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Molecular marker feedback simplifies the management of multi-trait breeding by providing clear genetic information about which desired traits are present in each progeny. This feedback system allows breeders to efficiently select for multiple traits simultaneously without the complexity of traditional multi-generational phenotypic selection and testing.

Inventive Principle:
Principle #23Feedback

Data Source

PatentUS9585331B2Soybean variety 01059966
Publication Date: 2017.03.07 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01059966. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01059966. Also provided by the invention are tissue cultures of the soybean variety 01059966 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01059966 with itself or another soybean variety and plants produced by such methods.