Soybean Variety 01064143 Genetic Transformation

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with desirable traits such as disease resistance, drought tolerance, and improved nutritional quality, as they rely on conventional breeding techniques that are time-consuming and unpredictable, and often result in varieties that are difficult to replicate.

Innovation Solution

The development of the soybean variety 01064143, which incorporates specific genetic loci conferring traits like herbicide resistance, disease resistance, and improved nutritional quality through genetic transformation and backcrossing, allowing for the creation of stable and high-yielding soybean plants.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding techniques are used to develop soybean varieties, then varieties can be developed with desirable traits, but the process is time-consuming and unpredictable

Engineering Contradiction:
Improvepredictability of trait introductionVSAvoidbreeding development time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces conventional mechanical breeding methods (cross-pollination, selection, and recombination) with genetic transformation technology. This allows direct introduction of specific genetic loci into the soybean genome, eliminating the need for multiple generations of crossing and selection. The genetic transformation approach enables precise, predictable insertion of desired traits without relying on random recombination events, thereby resolving the contradiction between reliability and time consumption in variety development.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Stability of the object's composition

If conventional breeding techniques are used, then varieties can be developed, but they are difficult to replicate

Engineering Contradiction:
Improvevariety replicabilityVSAvoidbreeding efficiency
Core Design Contradiction:
Stability of the object's compositionVSProductivity

Solution Approach 1:

The patent employs preliminary action by first identifying and characterizing specific genetic loci associated with desirable traits (herbicide resistance, disease resistance, nutritional quality) before introducing them into the soybean variety. This pre-characterization ensures that the exact genetic components responsible for desired traits are known and can be consistently replicated. The use of molecular markers and genetic mapping allows breeders to track and confirm the presence of these loci, ensuring variety stability and replicability across different breeding programs and locations.

Inventive Principle:
Principle #10Preliminary action

3Adaptability or versatility

If multiple desirable traits are combined in a single variety, then the variety becomes more valuable, but the breeding process becomes more complex

Engineering Contradiction:
Improvenumber of desirable traitsVSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent merges multiple desirable traits into a single soybean variety through genetic transformation, combining herbicide resistance, disease resistance, and improved nutritional quality in one genetic package. Rather than separately breeding for each trait and then attempting to combine them through complex crossing programs, the transformation approach allows simultaneous introduction of multiple genetic loci. This merging strategy reduces breeding process complexity while achieving high adaptability and versatility in the final variety.

Inventive Principle:
Principle #5Merging (Combining)

4Productivity

If genetic transformation is used to introduce specific traits, then trait introduction becomes predictable and efficient, but the technology complexity increases

Engineering Contradiction:
Improvebreeding speedVSAvoidtechnology complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent uses molecular markers and genetic mapping data as intermediaries to bridge the gap between genetic transformation and phenotypic expression. These markers serve as detectable signals that confirm successful introduction and integration of desired genetic loci. The intermediary approach simplifies the overall process by providing clear, measurable endpoints for transformation success, reducing the need for complex phenotypic screening and selection procedures. This mediator strategy enables rapid, predictable trait introduction while managing technology complexity through standardized, marker-assisted protocols.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS9867356B2Soybean variety 01064143
Publication Date: 2018.01.16 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01064143. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01064143. Also provided by the invention are tissue cultures of the soybean variety 01064143 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01064143 with itself or another soybean variety and plants produced by such methods.