Soybean Variety 01064349 Herbicide Resistance Breeding

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are not efficiently combined in a single variety.

Innovation Solution

The development of the soybean variety 01064349, which incorporates traits like glyphosate tolerance and dicamba resistance through genetic transformation, along with advanced breeding techniques like backcrossing and marker-assisted selection, to produce plants with desired agronomic and compositional characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional breeding methods are used to develop soybean varieties, then traditional traits may be maintained, but it is difficult to efficiently combine multiple improved traits such as disease resistance, drought tolerance, and herbicide resistance in a single variety

Engineering Contradiction:
Improvecombination of improved traitsVSAvoidbreeding efficiency
Core Design Contradiction:
Adaptability or versatilityVSProductivity

Solution Approach 1:

The patent employs molecular markers as intermediaries to identify and select plants carrying desired traits. These markers serve as detectable indicators that mediate the selection process, allowing breeders to efficiently track and combine multiple traits (disease resistance, drought tolerance, herbicide resistance) without lengthy conventional breeding cycles. The markers enable indirect selection of target genes, significantly improving breeding efficiency.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent replaces traditional mechanical/phenotypic selection methods with molecular-based detection systems. Instead of visually assessing and manually selecting plants with desired traits through conventional breeding, the invention uses DNA markers and molecular assays to automatically identify and select plants carrying specific trait combinations, thereby substituting mechanical breeding processes with more efficient molecular techniques.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Reliability

If multiple traits are combined through conventional breeding, then variety improvement may be achieved, but the breeding process becomes time-consuming and complex

Engineering Contradiction:
Improvetrait stabilityVSAvoidbreeding cycle duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent performs preliminary identification of desired traits at the molecular level before actual breeding operations. By using molecular markers to pre-screen and identify plants carrying target traits (disease resistance, drought tolerance, herbicide resistance) early in the breeding process, the invention eliminates time-consuming trial-and-error breeding cycles. This preliminary molecular characterization allows for more direct and efficient trait combination.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent implements a feedback mechanism where molecular marker data continuously informs breeding decisions. The marker-based detection system provides real-time feedback on whether desired traits are present in breeding populations, allowing breeders to adjust selection strategies immediately. This feedback loop accelerates the breeding process by eliminating uncertainty and enabling data-driven decisions about which plants to propagate or cross.

Inventive Principle:
Principle #23Feedback

3Adaptability or versatility

If advanced breeding techniques like genetic transformation are used, then desired traits such as glyphosate tolerance and dicamba resistance can be introduced, but the complexity of the breeding program increases

Engineering Contradiction:
Improveherbicide resistanceVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent uses molecular markers as intermediaries to simplify the management of genetically transformed plants. Rather than dealing with the full complexity of genetic transformation and trait verification, the invention employs marker-based selection to track the presence of transformed genes and their associated traits (glyphosate tolerance, dicamba resistance). This intermediary approach reduces program complexity by providing a straightforward detection and selection system.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS10342197B2Soybean variety 01064349
Publication Date: 2019.07.09 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01064349. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01064349. Also provided by the invention are tissue cultures of the soybean variety 01064349 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01064349 with itself or another soybean variety and plants produced by such methods.