Soybean Variety 01064490 Trait Stacking via Backcrossing

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are not adequately addressed by existing technologies.

Innovation Solution

The development of the soybean variety 01064490, which incorporates specific genetic loci conferring traits like glyphosate tolerance, dicamba tolerance, and resistance to various diseases, through genetic transformation and backcrossing techniques, allowing for the combination of desirable traits in a single variety.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional soybean breeding methods are used, then traditional variety development proceeds, but the ability to combine multiple desirable traits (disease resistance, drought tolerance, herbicide resistance) in a single variety is insufficient

Engineering Contradiction:
Improvetrait combination capabilityVSAvoidyield improvement rate
Core Design Contradiction:
Adaptability or versatilityVSProductivity

Solution Approach 1:

The patent combines multiple desirable traits (glyphosate tolerance, dicamba tolerance, disease resistance, yield improvement) from different parental lines into a single soybean variety through systematic backcrossing and selection, creating a unified variety that expresses all desired characteristics simultaneously

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The patent performs preliminary genetic modification by introducing specific tolerance traits (glyphosate and dicamba) into parental lines before crossing, so that these traits are already present and can be combined with other traits through subsequent backcrossing and selection processes

Inventive Principle:
Principle #10Preliminary action

2Adaptability or versatility

If multiple backcrossing generations are performed to combine desirable traits, then trait combination improves, but breeding time and complexity increase

Engineering Contradiction:
Improvetrait combinationVSAvoidbreeding cycle duration
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent performs a limited number of backcrossing generations (BC3F4) rather than continuing until complete genetic recovery, achieving sufficient trait combination and stability without the excessive time required for complete genomic recovery, thus optimizing the balance between trait combination and breeding time

Inventive Principle:
Principle #16Partial or excessive action

3Manufacturing precision

If selection pressure is increased to improve yield and resistance traits, then variety quality improves, but plant population survival during breeding decreases

Engineering Contradiction:
Improvevariety quality uniformityVSAvoidplant population size
Core Design Contradiction:
Manufacturing precisionVSQuantity of substance

Solution Approach 1:

The patent applies selection pressure periodically at specific stages (BC1F2, BC2F2, BC3F4 generations) rather than continuously, allowing population recovery between selection events while still achieving the desired quality uniformity through repeated cycles of selection and population expansion

Inventive Principle:
Principle #19Periodic action

Data Source

PatentUS10117407B1Soybean variety 01064490
Publication Date: 2018.11.06 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01064490. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01064490. Also provided by the invention are tissue cultures of the soybean variety 01064490 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01064490 with itself or another soybean variety and plants produced by such methods.