Soybean Variety 01064571 Breeding with Marker-Assisted Selection

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, herbicide tolerance, and nutritional quality, which are difficult to predict and require extensive research efforts due to the complexity of genetic combinations and environmental factors.

Innovation Solution

The development of the soybean variety 01064571, which incorporates specific genetic loci for traits like herbicide resistance, disease resistance, and improved nutritional quality through genetic transformation and backcrossing, allowing for the creation of hybrid seeds and plants suitable for commercial production and further breeding.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding methods are used to develop soybean varieties with improved traits, then genetic diversity can be maintained, but the breeding process becomes extremely time-consuming and difficult to predict due to complex genetic combinations and environmental factors

Engineering Contradiction:
Improvepredictability of trait expressionVSAvoidbreeding cycle duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-selecting parental lines with known, stable genotypes for specific traits (herbicide resistance, disease resistance, nutritional quality) before crossing. This preliminary characterization of parental genomes enables more predictable outcomes in the F1 hybrid, reducing the time needed for trait validation in later breeding stages.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses molecular markers to copy or track specific genomic regions associated with desirable traits through generations. By using marker-assisted selection, breeders can reliably identify and propagate desired traits without extensive phenotypic testing, improving predictability and reducing breeding cycle time.

Inventive Principle:
Principle #26Copying

2Adaptability or versatility

If multiple traits are combined in a single soybean variety through extensive breeding, then agronomic quality improves, but the complexity of the breeding program and genetic analysis increases significantly

Engineering Contradiction:
Improvenumber of improved traitsVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent segments the breeding program into distinct modules: selecting parental lines for specific traits (herbicide resistance, disease resistance, nutritional quality), performing controlled crosses, and evaluating F1 hybrids for each trait separately. This segmentation allows complex multi-trait varieties to be developed through systematic, manageable steps rather than attempting to combine all traits simultaneously.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent employs molecular marker technology that serves multiple functions: identifying parental genotypes, tracking trait inheritance through generations, and validating F1 hybrid composition. This multi-functional approach simplifies the breeding program by using a single analytical toolset for multiple breeding objectives.

Inventive Principle:
Principle #6Universality (Multi-functionality)

3Reliability

If F1 hybrid seeds are produced through crossing different soybean varieties, then novel trait combinations are achieved, but the production and propagation process becomes more complex compared to traditional varieties

Engineering Contradiction:
Improveconsistency of trait expressionVSAvoidseed production ease
Core Design Contradiction:
ReliabilityVSEase of manufacture

Solution Approach 1:

The patent implements feedback mechanisms by using molecular markers to verify the genotype of F1 hybrid seeds before and after production. This feedback ensures that only seeds with the correct parental genotype combination are propagated, maintaining trait consistency while streamlining the seed production process through objective genetic verification rather than extensive phenotypic screening.

Inventive Principle:
Principle #23Feedback

Data Source

PatentUS10244706B2Soybean variety 01064571
Publication Date: 2019.04.02 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01064571. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01064571. Also provided by the invention are tissue cultures of the soybean variety 01064571 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01064571 with itself or another soybean variety and plants produced by such methods.