Soybean Variety 01064596 Breeding via Marker-Assisted Selection

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are difficult to predict and require extensive research efforts due to the complexity of genetic combinations and environmental factors.

Innovation Solution

The development of the soybean variety 01064596, which incorporates specific genetic loci conferring traits like herbicide resistance, disease resistance, and improved agronomic characteristics through genetic transformation and backcrossing, allowing for the production of seeds and plants with consistent morphological and physiological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional soybean breeding methods are used to develop new varieties with improved traits, then genetic diversity and trait combinations can be explored, but the process requires extensive research efforts and is difficult to predict due to complexity of genetic combinations and environmental factors

Engineering Contradiction:
Improvetrait combination diversityVSAvoidbreeding program duration
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by creating isogenic lines through repeated backcrossing before the final cross. This preliminary preparation of genetically uniform lines with specific traits (herbicide resistance, disease resistance) allows for predictable F1 hybrid performance, reducing the time and uncertainty associated with conventional breeding trials.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses molecular marker-assisted selection to copy and track specific genetic loci through generations. By using DNA markers to identify and select plants with desired traits, the breeding process becomes more predictable and efficient, reducing the time required compared to phenotypic selection alone.

Inventive Principle:
Principle #26Copying

2Reliability

If multiple traits are introduced through conventional breeding, then variety improvement is achieved, but the complexity of genetic combinations increases and environmental variability affects prediction accuracy

Engineering Contradiction:
Improvetrait expression consistencyVSAvoidgenetic combination complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent applies local quality by introducing specific traits at specific genetic loci through targeted backcrossing and selection. Each isogenic line is developed with particular local improvements (e.g., herbicide resistance at one locus, disease resistance at another) while maintaining uniformity elsewhere, reducing genetic complexity and improving predictability.

Inventive Principle:
Principle #3Local quality

Solution Approach 2:

The patent segments the breeding process into distinct stages: developing individual isogenic lines with specific traits, then combining them in controlled crosses. This segmentation allows each trait to be optimized independently before combination, reducing the complexity of managing multiple traits simultaneously.

Inventive Principle:
Principle #1Segmentation

3Productivity

If extensive breeding research is conducted to develop stable varieties, then improved agronomic traits are achieved, but the cost and resource requirements increase

Engineering Contradiction:
Improveseed yieldVSAvoidbreeding resource investment
Core Design Contradiction:
ProductivityVSQuantity of substance

Solution Approach 1:

The patent replaces traditional mechanical/phenotypic selection methods with molecular marker-assisted selection. By using DNA markers to identify plants with desired traits, the process becomes more efficient and requires fewer resources, reducing the quantity of breeding material and time needed to achieve the same productivity improvements.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS10251354B2Soybean variety 01064596
Publication Date: 2019.04.09 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01064596. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01064596. Also provided by the invention are tissue cultures of the soybean variety 01064596 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01064596 with itself or another soybean variety and plants produced by such methods.