Soybean Variety 01067553 Single Locus Conversion

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are difficult to predict and require extensive research efforts due to the complexity of genetic combinations and environmental factors.

Innovation Solution

The development of the soybean variety 01067553, which involves genetic modification through single locus conversion using transgenic genes and genome editing techniques, such as Cas endonucleases, to introduce traits like herbicide resistance, disease resistance, and improved nutritional quality, while maintaining the morphological and physiological characteristics of the original variety.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional soybean breeding methods are used to develop new varieties with improved traits, then genetic diversity and trait combination are achieved, but the breeding process becomes extremely complex and time-consuming with unpredictable results

Engineering Contradiction:
Improvetrait combinationVSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent segments the breeding process by focusing on single locus conversions rather than attempting to combine multiple traits simultaneously. This allows breeders to make precise, targeted genetic modifications at specific loci while maintaining the rest of the genome intact, thereby reducing overall breeding complexity while achieving desired trait combinations through sequential improvements

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent applies local quality by making specific genetic modifications at particular loci rather than throughout the entire genome. This targeted approach allows for precise introduction of desired traits (such as herbicide resistance or disease resistance) at specific genetic locations while preserving the overall genetic integrity and performance characteristics of the parent variety

Inventive Principle:
Principle #3Local quality

2Reliability

If extensive research efforts are invested in conventional breeding to achieve desired traits, then trait improvement is achieved, but the time required for variety development increases significantly

Engineering Contradiction:
Improvetrait improvementVSAvoidbreeding duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent employs preliminary action by using genome editing technologies to directly introduce desired traits through single locus conversions, bypassing the need for lengthy conventional breeding cycles. This allows breeders to make targeted genetic changes in advance and achieve reliable trait improvement much more quickly than traditional methods, reducing breeding duration from many years to a fraction of that time

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent replaces the mechanical system of conventional breeding (crossing, selection, and multiple generations of propagation) with a more efficient molecular biology-based system. By using genome editing tools to directly modify specific loci, the process substitutes complex mechanical breeding operations with precise molecular interventions, thereby achieving the same or better trait improvement in significantly less time

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Productivity

If multiple traits are combined in a single variety through conventional breeding, then variety performance is improved, but the difficulty of predicting and achieving desired trait combinations increases

Engineering Contradiction:
Improvevariety performanceVSAvoidtrait prediction difficulty
Core Design Contradiction:
ProductivityVSDifficulty of detecting and measuring

Solution Approach 1:

The patent segments the trait improvement process into discrete single locus conversions, making each genetic modification independent and predictable. This allows breeders to track and predict the effects of each individual locus conversion separately, then combine them in known ratios (such as 5:1 or 9:3:3:1) to achieve desired multi-trait varieties with predictable performance outcomes

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent applies parameter changes by using molecular markers and genetic analysis to precisely measure and track the presence and expression of specific traits. This allows for accurate detection and measurement of trait combinations, making it possible to predict and achieve desired variety performance through controlled crosses of lines with known genetic compositions

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Soybean variety 01067553 exhibits improved agronomic traits, including resistance to herbicides like glyphosate and dicamba, moderate tolerance to diseases, and enhanced nutritional quality, demonstrating a predictable and efficient approach to breeding high-yielding soybeans with desired characteristics.

Implementation Method 1

the single locus conversion may comprise a transgenic gene which has been introduced by genetic transformation into the soybean variety 01067553 or a progenitor thereof

Methodology Applied
Scientific EffectGenome editing:

Implementation Method 2

genome editing techniques, such as Cas endonucleases, to introduce traits

Methodology Applied
Scientific EffectEndonuclease activity:

Data Source

PatentUS10499602B1Soybean variety 01067553
Publication Date: 2019.12.10 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01067553. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01067553. Also provided by the invention are tissue cultures of the soybean variety 01067553 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01067553 with itself or another soybean variety and plants produced by such methods.