Soybean Variety 01068135 Breeding for Trait Stability

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are not efficiently combined in a single variety.

Innovation Solution

The development of the soybean variety 01068135, which incorporates traits like glyphosate tolerance and dicamba resistance through genetic transformation, along with specific breeding methods like backcrossing and marker-assisted selection, to produce plants with desired agronomic and compositional characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional breeding methods are used to develop soybean varieties, then traditional traits may be maintained, but the ability to efficiently combine multiple improved traits (disease resistance, drought tolerance, herbicide resistance) in a single variety is limited

Engineering Contradiction:
Improvetrait combination capabilityVSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent combines multiple breeding techniques (backcrossing, marker-assisted selection, and genetic transformation) into an integrated breeding program. This merging of methods enables the efficient combination of multiple traits including disease resistance, drought tolerance, and herbicide resistance in a single soybean variety, directly addressing the limitation of conventional breeding in trait combination capability

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

Molecular markers serve as intermediaries in the breeding process, allowing breeders to track and select for multiple traits simultaneously without directly observing each trait's expression. This intermediary approach simplifies the complexity of managing multiple trait combinations by providing a standardized selection mechanism

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If genetic transformation is used to introduce herbicide tolerance traits, then herbicide resistance is achieved, but the stability and uniformity of the variety may be compromised

Engineering Contradiction:
Improveherbicide tolerance efficiencyVSAvoidgenetic stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent performs preliminary stability testing and characterization of genetically transformed soybean varieties before commercial deployment. This includes multiple generations of field testing to ensure the transformed varieties maintain genetic stability and uniformity, thereby resolving the concern that genetic transformation compromises variety stability

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The breeding program incorporates feedback mechanisms where the performance and stability of genetically transformed varieties are continuously monitored across different environments and generations. This feedback allows for selection and refinement of the most stable lines, ensuring that herbicide tolerance is achieved without compromising genetic stability

Inventive Principle:
Principle #23Feedback

3Productivity

If multiple breeding techniques are integrated to achieve superior varieties, then trait combination and yield improvement are enhanced, but the breeding program complexity and resource requirements increase

Engineering Contradiction:
Improveyield improvementVSAvoidbreeding program complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The breeding program is segmented into distinct, modular stages: initial genetic transformation, backcrossing generations, marker-assisted selection phases, and final yield testing. This segmentation allows each technique to be applied systematically and independently, managing overall program complexity while achieving superior yield improvement through cumulative trait integration

Inventive Principle:
Principle #1Segmentation

Data Source

PatentUS10231405B2Soybean variety 01068135
Publication Date: 2019.03.19 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01068135. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01068135. Also provided by the invention are tissue cultures of the soybean variety 01068135 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01068135 with itself or another soybean variety and plants produced by such methods.