Soybean Variety 01068359 Breeding via Marker-Assisted Selection

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are difficult to predict and require extensive research efforts due to the complexity of genetic combinations and environmental factors.

Innovation Solution

The development of the soybean variety 01068359, which includes specific genetic modifications and breeding techniques like backcrossing and genetic transformation to introduce desirable traits like herbicide resistance, disease resistance, and improved agronomic characteristics, using methods like microprojectile bombardment and Agrobacterium-mediated transformation.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional breeding methods are used to develop soybean varieties with improved traits, then genetic diversity and trait combinations can be explored, but the breeding process becomes extremely time-consuming and unpredictable due to the complexity of genetic combinations and environmental factors

Engineering Contradiction:
Improvetrait improvementVSAvoidbreeding duration
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-selecting parental lines with desired traits (herbicide resistance, disease resistance, yield characteristics) before initiating the breeding program. This preliminary selection of germplasm with known trait profiles reduces the time required for trait introduction and stabilization in subsequent generations.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses molecular markers as intermediaries to track and select for desired traits during breeding. These markers serve as mediators between phenotypic selection and genotypic changes, enabling more efficient and predictable trait introduction without requiring extensive phenotypic evaluation across multiple environments.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Reliability

If multiple traits are introduced into soybean varieties through conventional breeding, then the variety becomes more resilient and productive, but the complexity of managing genetic combinations and environmental interactions increases significantly

Engineering Contradiction:
Improvevariety stabilityVSAvoidgenetic management complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent segments the breeding process into distinct phases: parental line selection, controlled crossing, molecular marker-assisted selection, and multi-location testing. This segmentation allows each trait to be introduced and evaluated systematically, reducing the complexity of managing multiple genetic combinations simultaneously.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent changes the parameter of selection methodology from traditional phenotypic selection to molecular marker-assisted selection. This parameter change enables more precise tracking of desired traits through generations, reducing the complexity of managing genetic combinations and environmental interactions.

Inventive Principle:
Principle #35Parameter changes

3Productivity

If extensive research efforts are devoted to developing high-yielding soybean varieties, then grain production and food supply are maximized, but the resources and time required for breeding increase

Engineering Contradiction:
Improvegrain yieldVSAvoidresearch duration
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The patent replaces mechanical/phenotypic selection methods with molecular marker-assisted selection. This substitution accelerates the identification of plants with desired yield-related genes, reducing the time required for breeding high-yielding varieties while maintaining the goal of maximizing grain production.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent uses molecular markers as copies or proxies for complex genetic traits. Instead of evaluating entire phenotypes across multiple environments, researchers can track specific marker alleles that correlate with desired yield characteristics, significantly reducing the time and resources required for variety development.

Inventive Principle:
Principle #26Copying

Data Source

PatentUS10582687B1Soybean variety 01068359
Publication Date: 2020.03.10 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01068359. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01068359. Also provided by the invention are tissue cultures of the soybean variety 01068359 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01068359 with itself or another soybean variety and plants produced by such methods.