Soybean 01068427 Marker-Assisted Trait Stacking

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, herbicide tolerance, and nutritional quality, which are difficult to predict and require extensive research efforts due to the complexity of genetic combinations and environmental factors.

Innovation Solution

The development of the soybean variety 01068427, which includes specific genetic modifications and locus conversions for traits like herbicide resistance, disease resistance, and improved nutritional quality, achieved through genetic transformation and backcrossing techniques, allowing for the introduction of desired traits while maintaining the original variety's morphological and physiological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop soybean varieties with improved traits, then genetic diversity is increased, but the breeding process becomes time-consuming and unpredictable due to complex genetic combinations and environmental factors

Engineering Contradiction:
Improvetrait improvementVSAvoidbreeding duration
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-selecting parental lines with desired traits (herbicide resistance, disease resistance, nutritional quality) and using molecular marker-assisted selection to identify and track specific genetic loci throughout the breeding process. This preliminary preparation and monitoring accelerates the breeding process by focusing on specific target traits rather than relying on random genetic recombination alone.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent employs feedback mechanisms through molecular marker-assisted selection, where DNA markers are used to continuously monitor and identify plants carrying desired genetic traits across multiple generations. This feedback allows breeders to make informed selection decisions at each stage, accelerating the development of varieties with improved traits while reducing the time required compared to traditional phenotypic selection alone.

Inventive Principle:
Principle #23Feedback

2Reliability

If multiple traits are combined in a single soybean variety, then agronomic performance is improved, but the genetic complexity and difficulty of breeding increases

Engineering Contradiction:
Improveagronomic performanceVSAvoidgenetic complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by breaking down the complex task of combining multiple traits into manageable components. Specific parental lines are selected for individual traits (herbicide resistance, disease resistance, nutritional quality), and molecular markers are used to track each trait independently through the breeding process. This segmented approach to trait incorporation reduces the overall genetic complexity by focusing on specific loci rather than attempting to manage all genetic variations simultaneously.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent employs parameter changes by using molecular marker data to transform the breeding process from phenotypic selection to genotypic selection. This parameter change allows for precise tracking of specific genetic loci associated with desired traits, enabling the combination of multiple traits while managing genetic complexity through marker-assisted selection rather than relying on traditional, more complex phenotypic evaluation methods.

Inventive Principle:
Principle #35Parameter changes

3Stability of the object's composition

If extensive research efforts are invested in soybean breeding, then variety stability and yield are improved, but resource consumption and breeding costs increase

Engineering Contradiction:
Improvevariety stabilityVSAvoidresource consumption
Core Design Contradiction:
Stability of the object's compositionVSQuantity of substance

Solution Approach 1:

The patent applies mechanics substitution by replacing traditional, labor-intensive phenotypic selection methods with molecular marker-assisted selection. Instead of manually evaluating and selecting plants based on physical characteristics across multiple generations, DNA markers are used to identify and track specific genetic loci associated with desired traits. This substitution reduces resource consumption in terms of labor, time, and field space while maintaining or improving variety stability and yield through more precise selection.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent employs copying by using molecular markers as genetic copies or signatures that represent specific traits of interest. These markers serve as surrogate indicators that can be easily detected and tracked in the laboratory, replacing the need for extensive field trials and phenotypic evaluations. This copying approach reduces resource consumption by allowing breeders to screen large numbers of plants for desired traits using simple molecular assays rather than conducting resource-intensive multi-year field tests.

Inventive Principle:
Principle #26Copying

Data Source

PatentUS20200060140A1Soybean variety 01068427
Publication Date: 2020.02.27 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01068427. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01068427. Also provided by the invention are tissue cultures of the soybean variety 01068427 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01068427 with itself or another soybean variety and plants produced by such methods.