Soybean Variety 01069676 Breeding with Marker-Assisted Selection

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, herbicide tolerance, and nutritional quality, which are difficult to predict and require extensive research and time due to the complexity of genetic combinations and environmental factors.

Innovation Solution

The development of the soybean variety 01069676, which includes specific genetic modifications and breeding techniques like backcrossing and genetic transformation to introduce traits like herbicide resistance, disease resistance, and improved nutritional quality, using methods such as microprojectile bombardment and Agrobacterium-mediated transformation, while maintaining the variety's morphological and physiological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding methods are used to develop soybean varieties with improved traits, then genetic diversity and trait combination are achieved, but the process requires extensive time and resources with unpredictable outcomes

Engineering Contradiction:
Improvepredictability of trait expressionVSAvoidbreeding development time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-selecting parental lines with desired traits (herbicide resistance, disease resistance, nutritional quality) and using molecular markers to identify and track specific genetic loci before and during breeding. This allows breeders to predictably introgress target traits into elite backgrounds through backcrossing, reducing the time and uncertainty associated with conventional breeding by knowing in advance which plants carry the desired genetic modifications.

Inventive Principle:
Principle #10Preliminary action

2Adaptability or versatility

If multiple traits are combined in a single soybean variety, then agronomic performance and resistance are improved, but the genetic complexity and breeding difficulty increase

Engineering Contradiction:
Improvenumber of traits per varietyVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by dividing the breeding process into distinct stages: first developing parental lines with specific individual traits (herbicide resistance, disease resistance, nutritional quality), then systematically combining these traits through controlled backcrossing. Molecular markers are used to track and segment the inheritance of each trait independently, allowing complex multi-trait varieties to be developed through manageable sequential steps rather than attempting to combine all traits simultaneously.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent applies universality by developing a standardized backcrossing protocol with molecular marker-assisted selection that can be universally applied to introgress any desired trait into any elite soybean background. The same methodological framework (marker selection, backcrossing generations, trait validation) is used regardless of which specific traits are being combined, making the breeding program scalable and adaptable to different trait combinations without requiring entirely different approaches for each case.

Inventive Principle:
Principle #6Universality (Multi-functionality)

3Manufacturing precision

If molecular marker-assisted selection and genetic transformation are used, then breeding precision and trait introduction are improved, but the technical complexity and cost increase

Engineering Contradiction:
Improvetrait introduction precisionVSAvoidbreeding technology complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent applies the intermediary principle by using molecular markers as mediators between the visible phenotypic traits and the underlying genetic loci. These markers serve as detectable intermediaries that allow breeders to indirectly select for desired traits through DNA analysis rather than waiting for phenotypic expression. This intermediary approach enables precise tracking of trait inheritance through backcrossing generations, achieving high breeding precision while using relatively simple and cost-effective marker-based assays rather than complex genomic editing technologies.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS10631505B2Soybean variety 01069676
Publication Date: 2020.04.28 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01069676. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01069676. Also provided by the invention are tissue cultures of the soybean variety 01069676 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01069676 with itself or another soybean variety and plants produced by such methods.