Soybean Variety 01072332 Trait Stacking via Genetic Transformation

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are not efficiently combined in a single variety.

Innovation Solution

The development of the soybean variety 01072332, which incorporates specific genetic modifications and locus conversions, including transgenic genes for herbicide resistance and disease resistance, achieved through genetic transformation and backcrossing, allowing for the combination of desirable traits like glyphosate and dicamba tolerance.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional soybean breeding methods are used to develop varieties with multiple improved traits, then the breeding process becomes increasingly complex and time-consuming, but the ability to combine multiple desirable traits (disease resistance, drought tolerance, herbicide resistance) in a single variety is limited

Engineering Contradiction:
Improvecombination of desirable traitsVSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent combines multiple desirable traits (glyphosate resistance, dicamba resistance, disease resistance, drought tolerance) into a single soybean variety through genetic transformation and backcrossing, merging functions that would traditionally require separate breeding programs into one unified variety

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The patent uses molecular markers as intermediaries to track and select for multiple traits simultaneously during breeding, and employs transgenic events as mediators to introduce multiple resistance traits through controlled genetic transformation rather than traditional lengthy crossing programs

Inventive Principle:
Principle #24Intermediary (Mediator)

2Adaptability or versatility

If multiple backcrossing generations are performed to combine traits from different parental lines, then trait combination improves, but the time required for variety development increases significantly

Engineering Contradiction:
Improvetrait combinationVSAvoidvariety development time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent performs preliminary genetic transformation to introduce multiple resistance traits into a single genomic background before beginning the backcrossing program, rather than introducing traits during the lengthy backcrossing process, thereby reducing the overall development time

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses molecular marker-assisted selection to provide feedback on the presence and combination of multiple traits during each backcrossing generation, allowing breeders to make informed selection decisions that efficiently combine desired traits while monitoring genetic recovery of the recurrent parent

Inventive Principle:
Principle #23Feedback

3Productivity

If traditional breeding methods are used to achieve high yield stability, then yield potential can be improved, but resistance to multiple herbicides and diseases cannot be efficiently incorporated

Engineering Contradiction:
Improveyield stabilityVSAvoidresistance to herbicides and diseases
Core Design Contradiction:
ProductivityVSAdaptability or versatility

Solution Approach 1:

The patent changes the genetic parameters of the soybean variety by introducing transgenic events that confer multiple herbicide resistances (glyphosate, dicamba) and disease resistances, thereby altering the variety's response to environmental stressors and improving both yield stability and resistance profile simultaneously

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS11109552B2Soybean variety 01072332
Publication Date: 2021.09.07 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01072332. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01072332. Also provided by the invention are tissue cultures of the soybean variety 01072332 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01072332 with itself or another soybean variety and plants produced by such methods.