Soybean Variety 01073069 Breeding via Isogenic Lines
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Solution Overview
Problem
Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are difficult to predict and require extensive research and time due to the unpredictability of genetic combinations in conventional breeding.
Innovation Solution
The development of the soybean variety 01073069, which includes specific genetic modifications and locus conversions, such as those conferring herbicide resistance and disease tolerance, achieved through genetic transformation and backcrossing techniques, allowing for the introduction of desired traits while maintaining the overall morphological and physiological characteristics of the soybean.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional breeding methods are used to develop soybean varieties with improved traits, then genetic diversity and trait combinations can be explored, but the process requires extensive research and time due to unpredictability of genetic combinations
Solution Approach 1:
The patent applies preliminary action by creating isogenic lines through repeated backcrossing before the final cross. This preparatory step ensures that the genetic background is standardized and predictable, allowing the desired traits to be introduced without the uncertainty of varying genetic backgrounds. The isogenic lines are prepared in advance with specific traits (herbicide resistance, disease resistance) already established, so that when crossed, the resulting variety has predictable trait expression.
Solution Approach 2:
The patent changes the genetic parameter by introducing specific locus conversions at defined positions in the genome. Instead of relying on random genetic recombination, the invention uses targeted genetic modifications at specific loci (such as the I locus for herbicide resistance) to introduce desired traits. This parameter change approach allows precise control over which traits are introduced while maintaining the isogenic background, reducing the unpredictability of conventional breeding.
2Reliability
If multiple traits are combined in a single variety through conventional breeding, then improved agronomic performance can be achieved, but the complexity of the breeding program increases
Solution Approach 1:
The patent applies segmentation by dividing the breeding program into distinct modular components: creating isogenic line A with trait set X, creating isogenic line B with trait set Y, and then crossing them to combine traits. Each isogenic line is developed independently with specific traits, and the final variety is assembled by combining these pre-characterized modules. This segmentation reduces program complexity by breaking down the complex task of multi-trait variety development into manageable, repeatable units.
Solution Approach 2:
The patent creates isogenic lines that serve multiple functions: they maintain a standardized genetic background for predictability, carry specific desired traits for agronomic improvement, and can be used repeatedly as parental lines to produce consistent offspring. The isogenic lines act as universal building blocks that can be crossed with different partners or used in multiple breeding programs, reducing the need to develop new parental lines for each breeding objective.
3Manufacturing precision
If genetic transformation and backcrossing techniques are used to introduce desired traits, then specific traits such as herbicide resistance and disease tolerance can be precisely introduced, but the genetic modification process adds complexity to the breeding method
Solution Approach 1:
The patent uses isogenic lines as intermediaries between the genetic transformation process and the final variety development. The complex genetic modification work (transformation and backcrossing) is performed once to create the isogenic lines with desired traits. These isogenic lines then serve as stable intermediary parents that can be crossed with other isogenic lines to produce the final variety. This intermediary approach consolidates the complexity into a one-time event rather than requiring complex modifications at each breeding stage.
Solution Approach 2:
The patent creates copies of the desired genetic modifications by developing isogenic lines that are genetically identical except for the introduced traits. Once a trait is successfully introduced into an isogenic line through transformation or backcrossing, that line serves as a template or copy that can be used repeatedly. The standardized genetic background is copied across multiple lines, each carrying different desired traits, allowing precise trait introduction without repeating the complex modification process for each variety.
Data Source
AI summary
The invention relates to the soybean variety designated 01073069. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01073069. Also provided by the invention are tissue cultures of the soybean variety 01073069 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01073069 with itself or another soybean variety and plants produced by such methods.