Soybean Variety 01073072 Immortalized Cell Line Breeding
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Solution Overview
Problem
Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are difficult to predict and require extensive research and time due to the unpredictability of genetic combinations in conventional breeding.
Innovation Solution
The development of the soybean variety 01073072, which includes specific genetic modifications and locus conversions, such as those conferring herbicide resistance and disease tolerance, achieved through genetic transformation and backcrossing techniques, allowing for the introduction of desired traits while maintaining the morphological and physiological characteristics of the original variety.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional breeding methods are used to develop soybean varieties with improved traits, then genetic diversity and trait combinations can be achieved, but the process requires extensive research and time due to the unpredictability of genetic combinations
Solution Approach 1:
The patent applies preliminary action by creating an immortalized hybrid cell line that preserves the complete genetic combination of the F1 hybrid in a stable, renewable cell culture system. This allows the genetic material to be maintained indefinitely without repeated crossing experiments, enabling researchers to study and select from predetermined trait combinations without the time-consuming process of generating new F2 segregating populations each time.
Solution Approach 2:
The patent employs copying by creating a cellular copy of the hybrid genome through tissue culture and cell suspension techniques. The immortalized cell line serves as a renewable copy of the hybrid genetic material, allowing multiple generations of research and selection without requiring repeated sexual reproduction and genetic recombination, thus preserving the original trait combination while eliminating the need for time-consuming breeding cycles.
2Reliability
If conventional breeding methods are used to achieve stable, high-yielding varieties, then genetic combinations can be explored, but the process is unpredictable and requires extensive research
Solution Approach 1:
The patent applies preliminary action by establishing a stable, immortalized cell line that locks in the desired genetic combination before variety development begins. This pre-stabilized cellular foundation provides a reliable starting point for variety multiplication and breeding, eliminating the unpredictability of F2 segregation and reducing the need for extensive screening and selection in later breeding stages.
Solution Approach 2:
The patent applies parameter changes by transitioning from sexual reproduction with its inherent genetic variability to asexual cellular propagation that maintains genetic stability. The immortalized cell line provides a consistent, clonal source of genetic material, changing the fundamental parameter of genetic transmission from meiotic recombination to mitotic replication, thereby ensuring variety stability while simplifying the breeding process.
3Adaptability or versatility
If genetic transformation and backcrossing techniques are used to introduce desired traits, then specific traits such as herbicide resistance and disease tolerance can be incorporated, but the process requires maintaining morphological and physiological characteristics of the original variety
Solution Approach 1:
The patent applies local quality by making targeted genetic modifications at specific loci within the soybean genome while preserving the rest of the genetic background. Through backcrossing and selection, the desired traits (herbicide resistance, disease tolerance) are introduced at specific genetic locations, while the overall morphological and physiological characteristics of the original variety are maintained in the surrounding genomic regions.
Solution Approach 2:
The patent employs copying by using the immortalized cell line as a stable template that replicates the desired genetic combination. This cellular copy ensures that the introduced traits are consistently propagated alongside the original variety's characteristic features, maintaining genetic fidelity across generations and ensuring that the modified variety retains the morphological and physiological properties of the parent while incorporating new traits.
Data Source
AI summary
The invention relates to the soybean variety designated 01073072. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01073072. Also provided by the invention are tissue cultures of the soybean variety 01073072 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01073072 with itself or another soybean variety and plants produced by such methods.