Soybean Variety 01073533 Single-Locus Conversion Breeding

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are difficult to predict and require extensive research efforts due to the complexity of genetic combinations and environmental factors.

Innovation Solution

The development of the soybean variety 01073533, which includes genetic modifications through single locus conversions using transgenic genes for herbicide resistance, disease resistance, and other desirable traits, achieved by techniques like backcrossing and genetic transformation, allowing for the introduction of specific traits while maintaining the morphological and physiological characteristics of the original variety.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding methods are used to develop soybean varieties with improved traits, then genetic diversity is maintained, but the development process is time-consuming and unpredictable due to complex genetic combinations

Engineering Contradiction:
Improvepredictability of trait inheritanceVSAvoidbreeding development time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent applies parameter changes by modifying the genetic composition of soybean varieties through controlled introduction of specific alleles at targeted loci. This allows predictable alteration of traits such as disease resistance and herbicide tolerance while maintaining the overall genetic background and performance characteristics of the parent variety, thereby reducing breeding time and improving predictability.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent segments the breeding process by focusing on individual loci rather than attempting to manage entire genomes. By making single-locus conversions at specific positions (e.g., introducing Rps1c allele for Phytophthora resistance or converting to herbicide-resistant alleles), the complex breeding problem is divided into manageable, predictable steps that can be executed independently and combined systematically.

Inventive Principle:
Principle #1Segmentation

2Adaptability or versatility

If multiple traits are combined in soybean breeding to improve disease resistance, drought tolerance, and herbicide resistance, then variety performance is enhanced, but the complexity of genetic management increases

Engineering Contradiction:
Improvemulti-trait performanceVSAvoidgenetic combination complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent applies local quality by introducing specific beneficial alleles at particular loci while maintaining the original genetic background elsewhere in the genome. For example, introducing the Rps1c allele specifically for Phytophthora resistance or the HPPD-resistant allele for herbicide tolerance, rather than attempting to redesign entire genetic pathways. This allows accumulation of multiple beneficial traits with minimal interaction complexity.

Inventive Principle:
Principle #3Local quality

Solution Approach 2:

The patent demonstrates universality by developing a standardized single-locus conversion platform that can be applied to introduce various different traits using the same methodological framework. The same approach (marker-assisted selection, controlled crossing, genomic evaluation) can be used to introduce disease resistance, herbicide tolerance, or other traits, making the system universally applicable across different breeding objectives.

Inventive Principle:
Principle #6Universality (Multi-functionality)

3Manufacturing precision

If extensive research efforts are invested in soybean breeding to achieve superior varieties, then trait improvement is possible, but resource efficiency decreases due to the exploratory nature of conventional breeding

Engineering Contradiction:
Improvetrait improvement precisionVSAvoidresearch resource efficiency
Core Design Contradiction:
Manufacturing precisionVSLoss of energy

Solution Approach 1:

The patent implements feedback through the use of molecular markers and genomic evaluation systems that provide real-time information about the presence and expression of desired traits during the breeding process. This allows breeders to select plants with the intended genetic modifications early in development, rather than waiting for phenotypic expression, thereby reducing the number of breeding cycles and resource investment required to achieve the desired trait precision.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent applies preliminary action by using marker-assisted selection and genomic prediction to identify and select plants with desired genetic modifications early in the breeding process, before phenotypic expression occurs. This allows researchers to eliminate unwanted genetic combinations early, focusing resources only on promising lines that are likely to succeed, thereby improving resource efficiency while maintaining precision.

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS11129346B2Soybean variety 01073533
Publication Date: 2021.09.28 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01073533. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01073533. Also provided by the invention are tissue cultures of the soybean variety 01073533 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01073533 with itself or another soybean variety and plants produced by such methods.