Soybean Variety 01077377 Genetic Modification Breeding

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are difficult to predict and require extensive research and time due to the complexity of genetic combinations and environmental factors.

Innovation Solution

The development of the soybean variety 01077377, which includes specific genetic modifications and locus conversions through techniques like backcrossing and genetic transformation, incorporating traits like herbicide resistance, disease resistance, and improved nutritional quality, using methods such as genome editing with Cas endonucleases and other nucleases to introduce desired genes.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional soybean breeding methods are used to develop new varieties with improved traits, then trait improvement (disease resistance, drought tolerance, herbicide resistance) can be achieved, but the process requires extensive research time and is difficult to predict due to genetic complexity

Engineering Contradiction:
Improvepredictability of trait improvementVSAvoidbreeding research time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces conventional mechanical breeding methods (crossing, selection, and evaluation) with genetic engineering techniques. Specifically, it uses isolated DNA sequences as molecular markers to directly identify and select plants with desired traits, substituting the slow, unpredictable mechanical process of traditional breeding with a precise, accelerated molecular approach that reduces both time and uncertainty in variety development

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent introduces DNA sequences and molecular markers as intermediary tools between the breeder and the target traits. These markers serve as detectable intermediaries that indicate the presence of desired genetic characteristics without requiring direct observation or lengthy field testing, thereby accelerating the selection process and improving predictability of trait inheritance

Inventive Principle:
Principle #24Intermediary (Mediator)

2Adaptability or versatility

If multiple traits are combined in a single soybean variety through breeding, then the agronomic quality and yield potential improve, but the genetic complexity increases making the breeding process more difficult to manage

Engineering Contradiction:
Improvecombination of agronomic traitsVSAvoidgenetic combination complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent segments the complex task of multi-trait breeding into manageable molecular components. By isolating and identifying specific DNA sequences associated with individual traits (disease resistance, herbicide resistance, yield characteristics), the patent allows breeders to select for multiple traits independently through marker-assisted selection, thereby simplifying the management of genetic complexity while achieving comprehensive trait combinations

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent develops a universal molecular marker system that can detect and select for multiple different traits simultaneously. The isolated DNA sequences serve as universal indicators that can be applied across various breeding programs to select for diverse agronomic characteristics, reducing the complexity of managing multiple trait combinations by providing a unified selection approach

Inventive Principle:
Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Soybean variety 01077377 achieves enhanced yield, disease resistance, and improved agronomic traits, allowing for more predictable and efficient breeding processes, reducing the time and cost associated with developing superior soybean varieties.

Implementation Method 1

genome editing with Cas endonucleases and other nucleases to introduce desired genes

Methodology Applied
Scientific EffectDNA cleavage by endonucleases:

Data Source

PatentUS11096359B2Soybean variety 01077377
Publication Date: 2021.08.24 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01077377. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01077377. Also provided by the invention are tissue cultures of the soybean variety 01077377 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01077377 with itself or another soybean variety and plants produced by such methods.