Soybean Variety 01077435 Genetic Modification for Trait Stability

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and enhanced nutritional quality, which are difficult to predict and require extensive research efforts due to the complexity of genetic combinations and environmental factors.

Innovation Solution

The development of the soybean variety 01077435, which incorporates specific genetic modifications through transgenic genes and genome editing techniques, such as Cas endonucleases, to confer traits like herbicide resistance, disease resistance, and modified fatty acid composition, along with traditional breeding methods like backcrossing and marker-assisted selection.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop soybean varieties with improved traits, then genetic diversity and trait combination are enhanced, but the breeding process becomes lengthy and unpredictable due to complex genetic combinations and environmental factors

Engineering Contradiction:
Improvetrait combinationVSAvoidbreeding process duration
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies parameter changes by using transgenic genes and genome editing techniques to directly modify specific genetic parameters (fatty acid composition, herbicide resistance, disease resistance) rather than relying on traditional cross-breeding. This allows precise control over trait expression and significantly reduces the time required to develop varieties with desired characteristics, while maintaining the ability to combine multiple traits through targeted genetic modification.

Inventive Principle:
Principle #35Parameter changes

2Manufacturing precision

If transgenic genes and genome editing techniques are used to confer specific traits, then breeding precision and trait stability are improved, but the complexity of genetic manipulation and regulatory requirements increase

Engineering Contradiction:
Improvegenetic modification precisionVSAvoidgenetic manipulation complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent replaces traditional mechanical breeding methods (cross-pollination, selection, and hybridization) with molecular-level genetic manipulation techniques including transgenic gene introduction and CRISPR-Cas9 genome editing. This substitution enables precise targeting of specific genes responsible for fatty acid composition and resistance traits, achieving exact genetic modifications without the uncertainty and time consumption of conventional breeding approaches.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Reliability

If extensive research efforts are devoted to predicting and selecting desirable genetic combinations, then variety stability and yield potential are improved, but research costs and resource requirements increase

Engineering Contradiction:
Improvevariety stabilityVSAvoidresearch resources
Core Design Contradiction:
ReliabilityVSQuantity of substance

Solution Approach 1:

The patent employs preliminary action by pre-introducing transgenic genes with known functions (such as fatty acid desaturase genes for modified oil composition and resistance genes for herbicide and disease tolerance) before variety development. This approach eliminates the need for extensive field testing and selection of multiple genetic combinations, as the desired traits are already encoded in the genetically modified parent lines, thereby reducing research time and resource requirements while ensuring trait stability.

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS10660294B2Soybean variety 01077435
Publication Date: 2020.05.26 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01077435. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01077435. Also provided by the invention are tissue cultures of the soybean variety 01077435 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01077435 with itself or another soybean variety and plants produced by such methods.