Soybean Variety 01081408 Breeding via Marker-Assisted Selection

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are difficult to predict and require extensive research and time due to the complexity of genetic combinations and environmental factors.

Innovation Solution

The development of the soybean variety 01081408, which involves backcrossing and genetic transformation to introduce specific traits like herbicide resistance, disease resistance, and improved agronomic characteristics, using techniques like genome editing and marker-assisted selection, allowing for the recovery of desired morphological and physiological characteristics along with new loci.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding methods are used to develop soybean varieties with improved traits, then genetic diversity and adaptability are maintained, but the breeding process requires extensive time and resources with unpredictable outcomes

Engineering Contradiction:
Improvepredictability of trait inheritanceVSAvoidbreeding cycle duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces conventional mechanical breeding methods (cross-pollination, selection, and random mating) with biotechnological methods including genetic transformation, genome editing, and marker-assisted selection. This substitution enables precise introduction of desired traits through direct genetic modification rather than relying on random genetic recombination, thereby improving predictability and reducing breeding time

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent uses molecular markers as intermediaries to track and select for desired genetic traits during breeding. These markers serve as detectable indicators that allow breeders to identify plants carrying specific genes of interest without waiting for phenotypic expression, enabling earlier and more accurate selection decisions

Inventive Principle:
Principle #24Intermediary (Mediator)

2Adaptability or versatility

If multiple traits are combined in a single soybean variety, then overall agronomic performance is improved, but the genetic complexity and difficulty of breeding increases

Engineering Contradiction:
Improvenumber of improved traitsVSAvoidgenetic combination complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent divides the complex task of combining multiple traits into separate, manageable genetic components. Each desired trait (herbicide resistance, disease resistance, yield improvement) is introduced or selected independently through targeted genetic transformation or marker-assisted selection, allowing systematic accumulation of individual trait improvements rather than attempting to breed all traits simultaneously

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent employs universal molecular markers and standardized genetic transformation protocols that can be applied across different soybean varieties and trait types. This universal approach allows the same methodological framework to be used for introducing diverse traits, simplifying the breeding process despite the increasing number of traits being combined

Inventive Principle:
Principle #6Universality (Multi-functionality)

3Reliability

If extensive phenotypic screening and field testing are conducted to ensure variety stability, then variety reliability is improved, but the resources and time required increase significantly

Engineering Contradiction:
Improvevariety stabilityVSAvoidbreeding efficiency
Core Design Contradiction:
ReliabilityVSProductivity

Solution Approach 1:

The patent performs preliminary genetic analysis using molecular markers early in the breeding process to identify and select plants carrying desired traits before conducting time-consuming field trials. This preliminary genetic screening reduces the number of candidates that need to undergo extensive phenotypic testing, thereby maintaining variety reliability while improving breeding efficiency

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent implements feedback mechanisms through marker-assisted selection where genetic information obtained from molecular markers continuously guides breeding decisions. This feedback loop allows breeders to make informed selections at each generation, ensuring desired traits are maintained and combined effectively, reducing the need for extensive later-stage testing

Inventive Principle:
Principle #23Feedback

Data Source

PatentUS11266107B2Soybean variety 01081408
Publication Date: 2022.03.08 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01081408. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01081408. Also provided by the invention are tissue cultures of the soybean variety 01081408 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01081408 with itself or another soybean variety and plants produced by such methods.