Soybean Variety 01083659 Multi-Trait Stacking via Backcrossing

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Solution Overview

Problem

Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are difficult to predict and require extensive research efforts due to the complexity of genetic combinations and environmental factors.

Innovation Solution

The development of the soybean variety 01083659, which involves a specific breeding history and genetic modifications, including the introduction of transgenic genes for herbicide resistance and disease tolerance, using techniques like backcrossing and genetic transformation to combine desirable traits into a single locus conversion.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop soybean varieties with improved traits, then genetic diversity and trait combinations can be explored, but the process requires extensive research efforts and time due to the complexity of genetic combinations and environmental factors

Engineering Contradiction:
Improvetrait combination capabilityVSAvoidbreeding development time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-introducing specific transgenic genes (MON89788, A5547-127, MON87708) into the soybean variety 01083659 during the breeding process. This allows the desired traits for herbicide resistance and disease tolerance to be established in advance through genetic transformation, rather than relying on traditional slow methods of selecting and combining these traits through multiple generations of crossing and screening. The backcrossing process also preliminarily establishes the genetic background before final variety development.

Inventive Principle:
Principle #10Preliminary action

2Reliability

If transgenic genes are introduced to achieve herbicide resistance and disease tolerance, then specific trait performance is improved, but the genetic modification process and trait stability prediction become more complex

Engineering Contradiction:
Improveherbicide resistance and disease toleranceVSAvoidgenetic modification complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by introducing each desired trait through separate, well-defined transgenic events (MON89788 for glyphosate resistance, A5547-127 for glufosinate resistance, MON87708 for dicamba tolerance). Each gene insertion is an independent, characterized event with known genetic mechanisms. The backcrossing process further segments the genome to recover the desired genetic background while retaining the specific transgenic loci, making the complex genetic modification process more manageable and predictable.

Inventive Principle:
Principle #1Segmentation

3Adaptability or versatility

If multiple transgenic events are combined in a single variety, then multiple traits are achieved simultaneously, but the genetic stability and trait expression consistency become more difficult to predict

Engineering Contradiction:
Improvemulti-trait capabilityVSAvoidgenetic trait stability
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The patent uses backcrossing as an intermediary process to stabilize the genetic composition of the multi-trait variety. By repeatedly crossing the transgenic parent with the desired recurrent parent and selecting progeny that retain the transgenic events while recovering the recurrent parent's genome, the backcrossing process acts as a mediator that reduces genetic background complexity and stabilizes trait expression. This intermediary step ensures that the multiple transgenic events are maintained in a stable genetic context.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS11528879B2Soybean variety 01083659
Publication Date: 2022.12.20 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the soybean variety designated 01083659. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01083659. Also provided by the invention are tissue cultures of the soybean variety 01083659 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01083659 with itself or another soybean variety and plants produced by such methods.