Soybean Variety 01091765 CRISPR Trait Stacking
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Solution Overview
Problem
Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are difficult to predict and require extensive research and time due to the unpredictability of genetic combinations in conventional breeding.
Innovation Solution
The development of the soybean variety 01091765, which includes specific genetic modifications and locus conversions for traits like herbicide resistance, disease resistance, and improved agronomic characteristics through backcrossing and genetic transformation, using techniques like CRISPR-Cas systems and Agrobacterium-mediated transformation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional breeding methods are used to develop soybean varieties with improved traits, then genetic diversity and trait combination are achieved, but the process requires extensive time and unpredictable results
Solution Approach 1:
The patent replaces conventional mechanical cross-breeding methods with CRISPR-Cas9 gene editing technology. This substitution enables precise, targeted genetic modifications to introduce desired traits (herbicide resistance, disease resistance, yield improvement) directly into the soybean genome, eliminating the unpredictable and time-consuming nature of traditional breeding while achieving reliable and consistent trait inheritance
Solution Approach 2:
The patent applies parameter changes by using CRISPR-Cas9 to make specific, precise modifications to the soybean genome at targeted locations. This allows for controlled introduction of desired traits with known inheritance patterns, transforming the unpredictable genetic combinations of conventional breeding into predictable, engineered genetic changes that significantly reduce breeding cycle time
2Adaptability or versatility
If multiple traits are combined in a single soybean variety through conventional breeding, then improved agronomic performance is achieved, but the complexity of the breeding program increases
Solution Approach 1:
The patent segments the complex task of introducing multiple traits into separate, independent CRISPR-Cas9 editing operations. Each desired trait (herbicide resistance, disease resistance, yield improvement) can be targeted and edited independently at specific genomic loci, then combined in a systematic manner. This segmentation reduces breeding program complexity by breaking down the multifaceted conventional breeding process into discrete, manageable genetic editing steps
Solution Approach 2:
The patent uses parameter changes by precisely controlling the location and nature of genetic modifications through CRISPR-Cas9. Multiple traits are introduced by making specific, targeted changes to different genes or regulatory elements in the soybean genome, allowing for systematic combination of traits without the complexity of traditional poly-cross breeding programs
3Loss of time
If genetic transformation techniques are used to introduce specific traits, then breeding time is reduced, but the complexity of the transformation process increases
Solution Approach 1:
The patent replaces complex traditional genetic transformation methods (Agrobacterium-mediated transformation, particle bombardment) with CRISPR-Cas9 gene editing. This substitution simplifies the overall process by enabling direct, precise genomic modifications without requiring complex transformation vectors, selectable markers, or lengthy regeneration protocols, thereby reducing both time and procedural complexity
Solution Approach 2:
The patent applies parameter changes by using CRISPR-Cas9 to make targeted, site-specific modifications to the soybean genome. This approach changes the fundamental parameter of genetic modification from random or vector-dependent insertion to precise, location-specific editing, reducing transformation complexity while accelerating breeding cycles
Data Source
AI summary
The invention relates to the soybean variety designated 01091765. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01091765. Also provided by the invention are tissue cultures of the soybean variety 01091765 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01091765 with itself or another soybean variety and plants produced by such methods.