Soybean Variety 01094823 CRISPR Breeding Predictability
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Solution Overview
Problem
Current soybean breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and herbicide resistance, which are difficult to predict and require extensive research and time due to the unpredictability of genetic combinations in conventional breeding.
Innovation Solution
The development of the soybean variety 01094823, which includes specific genetic modifications and locus conversions for traits like herbicide resistance, disease resistance, and improved agronomic characteristics through backcrossing and genetic transformation, using techniques like CRISPR-Cas systems and Agrobacterium-mediated transformation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional breeding methods are used to develop soybean varieties with improved traits, then genetic diversity and trait combination are achieved, but the process is unpredictable and requires extensive time and research
Solution Approach 1:
The patent replaces conventional mechanical breeding methods (cross-pollination, selection, and generation advancement) with genetic engineering techniques including Agrobacterium-mediated transformation and CRISPR-Cas genome editing. This substitution enables direct introduction of desired traits through genetic transformation rather than relying on unpredictable natural recombination, thereby improving predictability and reducing breeding time
Solution Approach 2:
The patent uses Agrobacterium tumefaciens as an intermediary vector to deliver desired genes into soybean plants. This intermediary system enables precise genetic modification by mediating the transfer of specific trait-conferring genes (such as herbicide resistance genes) into the plant genome, making the breeding process more predictable and controllable compared to conventional methods
2Adaptability or versatility
If multiple traits are combined in a single soybean variety through conventional breeding, then improved agronomic performance is achieved, but the complexity of breeding programs increases
Solution Approach 1:
The patent merges multiple desired traits into a single soybean variety through genetic transformation, combining herbicide resistance, disease resistance, and improved agronomic characteristics in one genetic modification process. This merging approach simplifies the breeding program by introducing multiple traits simultaneously rather than through multiple sequential breeding steps
Solution Approach 2:
The patent employs universal genetic transformation techniques (Agrobacterium-mediated transformation and CRISPR-Cas editing) that can introduce various different traits using the same basic methodology. This multi-functional approach allows the same breeding technology platform to deliver diverse traits including herbicide resistance, disease resistance, and agronomic improvements, reducing program complexity
Data Source
AI summary
The invention relates to the soybean variety designated 01094823. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01094823. Also provided by the invention are tissue cultures of the soybean variety 01094823 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01094823 with itself or another soybean variety and plants produced by such methods.